Histone Deacetylases

Methods and Protocols

  • Sibaji Sarkar

Part of the Methods in Molecular Biology book series (MIMB, volume 1436)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Class I, II, and, IV Histone Deacetylases

    1. Front Matter
      Pages 1-1
    2. Martin Marek, Tajith B. Shaik, Sylvie Duclaud, Raymond J. Pierce, Christophe Romier
      Pages 109-118
    3. John Powers, Maritza Lienlaf, Patricio Perez-Villarroel, Susan Deng, Tessa Knox, Alejandro Villagra et al.
      Pages 129-145
    4. Jie Chen, Eva Sahakian, John Powers, Maritza Lienlaf, Patricio Perez-Villarroel, Tessa Knox et al.
      Pages 147-165
  3. Class III Deacetylases

    1. Front Matter
      Pages 167-167
    2. Ana R. Gomes, Jay Sze Yong, Khai Cheng Kiew, Ebru Aydin, Mattaka Khongkow, Sasiwan Laohasinnarong et al.
      Pages 169-188
    3. Shaoping Ji, J. Ronald Doucette, Adil J. Nazarali
      Pages 189-199
    4. Andy W. C. Man, Bo Bai, Yu Wang
      Pages 201-211
    5. Minna Rahnasto-Rilla, Maija Lahtela-Kakkonen, Ruin Moaddel
      Pages 259-269
  4. Histone Deacetylase Inhibitors

    1. Front Matter
      Pages 279-279
    2. Heidi Olzscha, Mina E. Bekheet, Semira Sheikh, Nicholas B. La Thangue
      Pages 281-303
  5. Jie Chen, Eva Sahakian, John Powers, Maritza Lienlaf, Patricio Perez-Villarroel, Tessa Knox et al.
    Pages E1-E1
  6. Back Matter
    Pages 335-337

About this book


This volume provides different methodologies for all classes of histone deacetylases, which includes detailed procedures on Class I and II histone deacetylase inhibitors, SIRT inhibitors, and bromodomain inhibitors. Histone Deacetylases: Methods and Protocols is divided into four sections: Sections A and B describe methodologies used to detect the activity, function, or chromatin location of HDACs 1 through 11, with Section A discussing Class I and Section B discussing class II histone deacetylases; Section C focuses on the methodologies for cloning and characterizing the acetylation of SIRTs 1 through 7; and Section D describes methods related to histone deacetylase inhibitors. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Histone Deacetylases: Methods and Protocols, is a valuable resource for investigators working on epigenetics, molecular biology, and genetics.


epigenetics deacetylation of histones in the nucleus deacetylation of cytoplasmic signaling proteins sirtuins inhibitors (SIRTs) histone deacetylase (HDACs) inhibitors bromodomain inhibitors acetylation of SIRTs enzymes

Editors and affiliations

  • Sibaji Sarkar
    • 1
  1. 1.Boston University School of MedicineBostonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3665-6
  • Online ISBN 978-1-4939-3667-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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