© 2016

Gastrointestinal Physiology and Diseases

Methods and Protocols

  • Andrei I. Ivanov

Part of the Methods in Molecular Biology book series (MIMB, volume 1422)

Table of contents

  1. Front Matter
    Pages i-xv
  2. In Vitro and Ex Vivo Systems to Study Gastrointestinal Functions and Diseases

    1. Front Matter
      Pages 1-1
    2. Yoshiaki Maru, Kaoru Orihashi, Yoshitaka Hippo
      Pages 13-21
    3. Nina Bertaux-Skeirik, Jomaris Centeno, Rui Feng, Michael A. Schumacher, Ramesh A. Shivdasani, Yana Zavros
      Pages 23-31
    4. Sadasivan Vidyasagar, Gordon MacGregor
      Pages 49-61
    5. Daniel J. Kao, Jordi M. Lanis, Erica Alexeev, Douglas J. Kominsky
      Pages 63-75
    6. Daniel J. Kao, Jordi M. Lanis, Erica Alexeev, Douglas J. Kominsky
      Pages 77-87
  3. Imaging Analysis of the Gastrointestinal System In Vivo

    1. Front Matter
      Pages 101-101
    2. Alyssa K. Whitney, Eric L. Campbell
      Pages 115-126
    3. Aneesh Alex, Elia D. Tait Wojno, David Artis, Chao Zhou
      Pages 127-136
    4. Elias Gounaris, Yasushige Ishihara, Manisha Shrivastrav, David Bentrem, Terrence A. Barrett
      Pages 137-147
    5. Rei Mizuno, Yuji Kamioka, Yoshiharu Sakai, Michiyuki Matsuda
      Pages 149-160
  4. Isolation and Characterization of Intestinal Immune Cells

    1. Front Matter
      Pages 169-169
    2. Akihito Harusato, Duke Geem, Timothy L. Denning
      Pages 171-180

About this book


This volume provides a comprehensive collection of  classical and cutting edge protocols and techniques to examine the normal development and physiological functions of the gastrointestinal system and to model the most common digestive diseases. The chapters focus on diverse research topics including ex vivo systems to study gastrointestinal development and functions, in vivo imaging of the gastrointestinal tract, isolation and characterization of intestinal immune cells, and animal models of gastrointestinal inflammation and cancer. The Gastrointestinal Physiology and Diseases: Methods and Protocols book targets wide audience of physiologists, cell and developmental biologists, immunologists, and physician-scientists working in the field of gastroenterology and beyond. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Highly practical and clearly written, Gastrointestinal Physiology and Diseases: Methods and Protocols will serve both seasoned researchers as well as newcomers to the field and will provide a unique resource and expert guidance to modern laboratory techniques developed for examining normal functions and diseases of the gastrointestinal tract.


gastrointestinal system digestive diseases gut mucosa intestinal immune cells tumor induction tumor development biochemical alterations

Editors and affiliations

  • Andrei I. Ivanov
    • 1
  1. 1.Dept of Human and Molecular GeneticsVCU Institute of Molecular Medicine, VCURichmondUSA

Bibliographic information

  • Book Title Gastrointestinal Physiology and Diseases
  • Book Subtitle Methods and Protocols
  • Editors Andrei I. Ivanov
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-3601-4
  • Softcover ISBN 978-1-4939-8091-8
  • eBook ISBN 978-1-4939-3603-8
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XV, 353
  • Number of Illustrations 25 b/w illustrations, 62 illustrations in colour
  • Topics Molecular Medicine
  • Buy this book on publisher's site
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