The Mitotic Spindle

Methods and Protocols

  • Paul Chang
  • Ryoma Ohi

Part of the Methods in Molecular Biology book series (MIMB, volume 1413)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Methods Focused on Cytology

    1. Front Matter
      Pages 1-1
    2. Sai K. Balchand, Barbara J. Mann, Patricia Wadsworth
      Pages 3-14
  3. Methods Focused on Microtubules and the Mitotic Spindle

  4. Methods Focused on Kinetochores and the Kinetochore-Microtubule Interface

    1. Front Matter
      Pages 109-109
    2. Matthew D. D. Miell, Aaron F. Straight
      Pages 111-133
    3. Tatsuya Nishino, Tatsuo Fukagawa
      Pages 135-146
    4. Keith F. DeLuca, Jacob A. Herman, Jennifer G. DeLuca
      Pages 147-168
    5. Tae Yeon Yoo, Daniel J. Needleman
      Pages 169-186
  5. Methods Focused on the Spindle Pole

    1. Front Matter
      Pages 187-187
    2. Kimberly K. Fong, Beth Graczyk, Trisha N. Davis
      Pages 189-195
    3. Vladimir Joukov, Johannes C. Walter, Arcangela De Nicolo
      Pages 207-235
  6. Methods Focused on the Cellular Functions of Microtubule Motor Proteins

    1. Front Matter
      Pages 237-237
    2. Cindy Fonseca, Jason Stumpff
      Pages 253-262
    3. Moé Yamada, Tomohiro Miki, Gohta Goshima
      Pages 263-282
    4. Naowras Al-Obaidi, Johanna Kastl, Thomas U. Mayer
      Pages 283-299
  7. Novel Approaches to Study Spindle Function and Regulation

    1. Front Matter
      Pages 301-301
    2. Amy B. Emerman, Ashwini Jambhekar, Michael D. Blower
      Pages 303-324
    3. Adrian T. Saurin, Geert J. P. L. Kops
      Pages 333-347
    4. Mathew J. K. Jones, Prasad V. Jallepalli
      Pages 349-363
  8. The Mitotic Spindle and Cancer

    1. Front Matter
      Pages 365-365
    2. Elizabeth M. Shenk, Neil J. Ganem
      Pages 393-401
    3. Stefan Florian, Timothy J. Mitchison
      Pages 403-421
  9. Back Matter
    Pages 423-424

About this book


This volume includes a series of protocols focused on mitotic spindle assembly and function. The methods covered in this book feature a broad range of techniques from basic microscopy to the study of spindle physiologies relevant to cancer. These methods can be applied to diverse model systems that range from the cell-free Xenopus egg extract system to the moss Physcomitrella patens, in an effort to demonstrate the key contributions made by researchers using multiple model organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the cross-disciplinary efforts, such as ATP analogue sensitive inhibition of mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and informative, The Mitotic Spindle: Methods and Protocols, is a valuable resource for researchers who are new to mitosis or are already experts in the field.



cell division light microscopy electron microscopy microtubule function microtubule polymerization dynamics core cell cycle control mechanisms spindle functions relevant to cancer cell-free Xenopus egg extracts system

Editors and affiliations

  • Paul Chang
    • 1
  • Ryoma Ohi
    • 2
  1. 1.Massachusetts Institute of TechnoloCambridgeUSA
  2. 2.Dept of Cell & Developmental BiologVanderbilt UniversityNashvilleUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3540-6
  • Online ISBN 978-1-4939-3542-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site