© 2016

The Nuclear Envelope

Methods and Protocols

  • Sue Shackleton
  • Philippe Collas
  • Eric C. Schirmer

Part of the Methods in Molecular Biology book series (MIMB, volume 1411)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Nuclear Envelope Isolation

    1. Front Matter
      Pages 1-1
    2. Xiao Zhou, Kentaro Tamura, Katja Graumann, Iris Meier
      Pages 45-65
    3. Samson O. Obado, Mark C. Field, Brian T. Chait, Michael P. Rout
      Pages 67-80
  3. Nuclear Envelope Protein Interactions, Localization, and Dynamics

  4. Nuclear Envelope Interactions with the Cytoskeleton

    1. Front Matter
      Pages 209-209
    2. Flavia Autore, Catherine M. Shanahan, Qiuping Zhang
      Pages 211-220
    3. Gregory Fedorchak, Jan Lammerding
      Pages 241-254

About this book


This volume provides a wide range of protocols used in studying the nuclear envelope, with special attention to the experimental adjustments that may be required to successfully investigate this complex organelle in cells from various organisms. The Nuclear Envelope: Methods and Protocols is divided into five sections: Part I – Nuclear Envelope Isolation; Part II – Nuclear Envelope Protein Interactions, Localization, and Dynamics; Part III – Nuclear Envelope Interactions with the Cytoskeleton; Part IV – Nuclear Envelope-Chromatin Interactions; and Part V – Nucleo-Cytoplasmic Transport. Many of the modifications discussed in this book have only been circulated within laboratories that have conducted research in this field for many years. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and thorough, The Nuclear Envelope: Methods and Protocols is a timely resource for researchers who have joined this dynamic and rapidly growing field.


Nuclear Envelope transmembrane proteins structural and signaling networks cytoskeleton gene regulation cellular organelles membrane-spanning regions nuclear pore complex

Editors and affiliations

  • Sue Shackleton
    • 1
  • Philippe Collas
    • 2
  • Eric C. Schirmer
    • 3
  1. 1.Department of BiochemistryUniversity of LeicesterLeicesterUnited Kingdom
  2. 2.Institute of Basic Medical SciencesUniversity of OsloOsloNorway
  3. 3.Wellcome Trust Centre for Cell BiolUniversity of EdinburghEdinburghUnited Kingdom

Bibliographic information

  • Book Title The Nuclear Envelope
  • Book Subtitle Methods and Protocols
  • Editors Sue Shackleton
    Philippe Collas
    Eric C. Schirmer
  • Series Title Methods in Molecular Biology
  • Series Abbreviated Title Methods Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-3528-4
  • Softcover ISBN 978-1-4939-8068-0
  • eBook ISBN 978-1-4939-3530-7
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XIV, 523
  • Number of Illustrations 26 b/w illustrations, 77 illustrations in colour
  • Topics Cell Biology
  • Buy this book on publisher's site
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