Animal Coronaviruses

  • Leyi Wang

Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Leyi Wang, Yan Zhang
      Pages 3-11
  3. Conventional Technologies

    1. Front Matter
      Pages 13-13
    2. Yi-Ning Chen, Ching Ching Wu, Tsang Long Lin
      Pages 25-32
    3. Chien Chang Loa, Ching Ching Wu, Tsang Long Lin
      Pages 33-42
  4. Reverse Genetics

    1. Front Matter
      Pages 51-51
    2. Sarah M. Keep, Erica Bickerton, Paul Britton
      Pages 53-72
  5. Molecular Diagnostics

About this book


This detailed volume provides diagnosticians and researchers with practical methodologies and approaches to tackle animal coronaviruses. It explores conventional immunohistochemistry, virus neutralization, enzyme-linked immunosorbent assays, expression and purification of recombinant viral proteins, and various molecular assays, including conventional and real-time reverse transcription-PCR, reverse genetics methodology, and next generation sequencing and sequence analyses. As part of the Springer Protocols Handbooks series, chapters contain readily reproducible laboratory protocols as well as expert tips on troubleshooting and avoiding known pitfalls. 

Practical and authoritative, Animal Coronaviruses serves as an ideal reference for researchers examining a wide variety of coronavirus species in the Coronaviridae.


Coronaviridae Reverse genetics Molecular diagnostics Next generation sequencing Virology Immunohistochemistry

Editors and affiliations

  • Leyi Wang
    • 1
  1. 1.Animal Disease Diagnostic LabOhio Department of AgricultureReynoldsburgUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3412-6
  • Online ISBN 978-1-4939-3414-0
  • Series Print ISSN 1949-2448
  • Series Online ISSN 1949-2456
  • About this book
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