Kidney Research

Experimental Protocols

  • Tim D. Hewitson
  • Edward R. Smith
  • Stephen G. Holt

Part of the Methods in Molecular Biology book series (MIMB, volume 1397)

Table of contents

  1. Front Matter
    Pages i-xi
  2. In Vitro, In Vivo and Ex Vivo Models

    1. Front Matter
      Pages 1-1
    2. Bart Smeets, Nazanin Kabgani, Marcus J. Moeller
      Pages 3-10
    3. Sven-Jean Tan, Tim D. Hewitson
      Pages 11-23
    4. Masahiro Eriguchi, Kazuhiko Tsuruya
      Pages 45-52
    5. Mei Jin, Yu Yaling, Wang Zhibin, Zhang Jianse
      Pages 53-63
  3. Imaging Techniques

    1. Front Matter
      Pages 65-65
    2. Kevin M. Bennett, Scott C. Beeman, Edwin J. Baldelomar, Min Zhang, Teresa Wu, Bradley D. Hann et al.
      Pages 67-79
    3. Muhammad Imtiaz Ashraf, Dietmar Fries, Werner Streif, Felix Aigner, Paul Hengster, Jakob Troppmair et al.
      Pages 81-90
  4. In Vivo Analysis of Metabolism in Ischemia-Reperfusion

    1. Front Matter
      Pages 91-91
    2. Maarten P. Koeners, Connie P. C. Ow, David M. Russell, Roger G. Evans, Simon C. Malpas
      Pages 93-111
    3. Andreas Pohlmann, Karen Arakelyan, Erdmann Seeliger, Thoralf Niendorf
      Pages 113-127
    4. Kathleen Cantow, Karen Arakelyan, Erdmann Seeliger, Thoralf Niendorf, Andreas Pohlmann
      Pages 129-154
    5. David M. Small, Washington Y. Sanchez, Glenda C. Gobe
      Pages 155-172
  5. Vascular Mineralization

  6. Analytical Techniques

    1. Front Matter
      Pages 249-249
    2. Tim D. Hewitson, Michael Christie, Edward R. Smith
      Pages 251-265
    3. Takahiro Horinouchi, Koji Terada, Tsunehito Higashi, Soichi Miwa
      Pages 267-277
    4. Katherine Ververis, Selly Marzully, Chrishan S. Samuel, Tim D. Hewitson, Tom C. Karagiannis
      Pages 279-289
  7. Back Matter
    Pages 291-292

About this book


This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification. The book is divided into five parts: Part I provides a number of in vitroin vivo, and ex vivo models of kidney disease and associated complications; Part II looks at recent advances in imaging techniques; Part III covers recent developments in studying metabolism in renal ischemia and reperfusion; Part IV addresses study and measurement of vascular calcification; and Part V explores analytical techniques that are both topical and of widespread relevance to the study of experimental renal disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Second Edition is a valuable collection of protocols useful to new and experienced researchers who are interested in the field of Nephrology.


renal anatomy renal physiology peritoneal dialysis cardio-renal syndrome vascular calcification glomerular

Editors and affiliations

  • Tim D. Hewitson
    • 1
  • Edward R. Smith
    • 2
  • Stephen G. Holt
    • 3
  1. 1.Royal Hospital Melbourne Dept. NephrologyMelbourneAustralia
  2. 2.Department of NephrologyRoyal Melbourne HospitalParkville, MelbourneAustralia
  3. 3.Department of NephrologyRoyal Melbourne HospitalParkvilleAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3351-8
  • Online ISBN 978-1-4939-3353-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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