© 2016

Advances and Clinical Practice in Pyrosequencing

  • Guohua Zhou
  • Qinxin Song


  • Provides a comprehensive description of current techniques and clinical applications of pyrosequencing

  • Addresses both the technical and clinical aspects of pyrosequencing uses

  • Describes the current applications of pyrosequencing in different fields, emphasizing their practicality and utility in clinical treatment, especially gene-guided personalized medicine

  • Provides technical protocols to measure SNPs or mutations, mRNA and microRNA quantification, as well as resequencing by pyrosequencing


Part of the Springer Protocols Handbooks book series (SPH)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Technology Preparation

    1. Front Matter
      Pages 1-1
    2. Huiyong Yang, Chao Liang, Zhiyao Chen, Bingjie Zou, Qinxin Song, Guohua Zhou
      Pages 3-12
    3. Yunlong Liu, Haiping Wu, Hui Ye, Zhiyao Chen, Qinxin Song, Bingjie Zou et al.
      Pages 13-21
    4. Qinxin Song, Huiyong Yang, Bingjie Zou, Hideki Kambara, Guohua Zhou
      Pages 23-30
    5. Qinxin Song, Haiping Wu, Guohua Zhou, Hideki Kambara
      Pages 31-39
    6. Huning Jia, Zhiyao Chen, Haiping Wu, Hui Ye, Bingjie Zou, Qinxin Song et al.
      Pages 41-49
    7. Pengfeng Xiao, Huan Huang, Bingjie Zou, Qinxin Song, Guohua Zhou, Zuhong Lu
      Pages 51-65
    8. Zhiyao Chen, Yunlong Liu, Hui Ye, Haiping Wu, Jinheng Li, Bingjie Zou et al.
      Pages 67-76
  3. Template Innovation

    1. Front Matter
      Pages 77-77
    2. Guohua Zhou, Tomoharu Kajiyama, Mari Gotou, Akihiko Kishimoto, Shigeya Suzuki, Hideki Kambara
      Pages 79-94
    3. Guohua Zhou, Masao Kamahori, Kazunori Okano, Gao Chuan, Kunio Harada, Hideki Kambara
      Pages 95-114
    4. Guohua Zhou, Hiromi Shirakura, Masao Kamahori, Kazunori Okano, Keiichi Nagai, Hideki Kambara
      Pages 115-128
    5. Guohua Zhou, Mari Gotou, Tomoharu Kajiyama, Hideki Kambara
      Pages 129-143
    6. Haiping Wu, Wenjuan Wu, Zhiyao Chen, Weipeng Wang, Bingjie Zou, Qinxin Song et al.
      Pages 145-154
    7. Qinxin Song, Xiemin Qi, Yiyun Shen, Yiru Jin, Bingjie Zou, Guohua Zhou
      Pages 155-166
  4. Reagents Preparation

    1. Front Matter
      Pages 167-167
    2. Shuhui Zhu, Bingjie Zou, Haiping Wu, Yinjiao Ma, Ying Chen, Qinxin Song et al.
      Pages 169-177

About this book


The aim of this book is to improve pyrosequencing protocols as well as instrumentation for better clinical use by describing improvements and novel applications of pyrosequencing technology.  Divided into five parts, the book’s thirty chapters explore advances in pyrosequencing template preparation, pyrosequencing technology innovations, multiplex pyrosequencing based on barcodes, the miniaturization of pyrosequencing equipment, as well as various applications. As part of the Springer Protocols program, chapters contain the kind of detail and practical implementation advice to guarantee successful results in the lab.

Comprehensive and thorough, Advances and Clinical Practice in Pyrosequencing serves as a valuable reference for researchers who are engaged in personalized medicine, disease control, and DNA diagnosis in numerous other fields.


Gene expression Personalized medicine Pharmacogenomics Polymerase chain reaction Single nucleotide polymorphism Template preparation Multiplex sequencing Equipment miniaturization Pyrosequencer

Editors and affiliations

  • Guohua Zhou
    • 1
  • Qinxin Song
    • 2
  1. 1.State Key Laboratory of Analytical ChemiMedical School of Nanjing UniversityNanjingChina
  2. 2.Key Laboratory of Drug Quality ControlChina Pharmaceutical UniversityNanjingChina

Bibliographic information

  • Book Title Advances and Clinical Practice in Pyrosequencing
  • Editors Guohua Zhou
    Qinxin Song
  • Series Title Springer Protocols Handbooks
  • Series Abbreviated Title Springer Protocols Handbooks
  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-3306-8
  • Softcover ISBN 978-1-4939-8017-8
  • eBook ISBN 978-1-4939-3308-2
  • Series ISSN 1949-2448
  • Series E-ISSN 1949-2456
  • Edition Number 1
  • Number of Pages XIV, 407
  • Number of Illustrations 44 b/w illustrations, 112 illustrations in colour
  • Topics Human Genetics
  • Buy this book on publisher's site
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