Methods and Protocols

  • Leonardo Salmena
  • Vuk Stambolic

Part of the Methods in Molecular Biology book series (MIMB, volume 1388)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introductory Chapters

    1. Front Matter
      Pages 1-1
    2. Leonardo Salmena
      Pages 3-11
    3. Olena Gorbenko, Vuk Stambolic
      Pages 13-19
  3. Methods to Detect PTEN in Disease

    1. Front Matter
      Pages 21-21
    2. Mireia Castillo-Martin, Tin Htwe Thin, Ana Collazo Lorduy, Carlos Cordon-Cardo
      Pages 23-37
    3. John F. Woolley, Leonardo Salmena
      Pages 39-51
    4. Guo Zhu, Suzanne J. Baker
      Pages 53-62
    5. Cynthia T. Luk, Stephanie A. Schroer, Minna Woo
      Pages 75-91
  4. Methods to Study PTEN Regulation

    1. Front Matter
      Pages 93-93
    2. Stanislas Misticone, Evelyne Lima-Fernandes, Mark G. H. Scott
      Pages 95-110
    3. Andrea Tuccoli, Marianna Vitiello, Andrea Marranci, Francesco Russo, Laura Poliseno
      Pages 111-138
    4. Yvonne Tay, Pier Paolo Pandolfi
      Pages 139-154
    5. Amit Gupta, Helene Maccario, Nisha Kriplani, Nicholas R. Leslie
      Pages 155-165
  5. Methods to Study PTEN Localization

    1. Front Matter
      Pages 167-167
    2. Anabel Gil, José I. López, Rafael Pulido
      Pages 169-186
    3. Sonia Missiroli, Claudia Morganti, Carlotta Giorgi, Paolo Pinton
      Pages 187-212
  6. Methods to Study PTEN Structure

    1. Front Matter
      Pages 213-213
  7. Methods to Study PTEN and Stem Cell Function

    1. Front Matter
      Pages 231-231
    2. Suzanne Schubbert, Jing Jiao, Marcus Ruscetti, Jonathan Nakashima, Shumin Wu, Hong Lei et al.
      Pages 233-285
  8. Methods to Study PTEN Using Animal Models

    1. Front Matter
      Pages 287-287
    2. Antonella Papa, Pier Paolo Pandolfi
      Pages 289-306
    3. Shanqing Zheng, Ian D. Chin-Sang
      Pages 307-321
  9. Back Matter
    Pages 323-324

About this book


This volume provides detailed descriptions of prevailing and novel techniques used by experts in the study of PTEN function in disease and biology. The book begins with chapters exploring methods to detect expression levels of PTEN in normal and diseased human specimens; methods to evaluate specific PTEN function in brain cancer; methods that utilize a new biosensor to measure PTEN regulation; and techniques to measure post-transcriptional regulation of PTEN by micoRNAs and ceRNAs. Other chapters present methods describing novel techniques to detect PTEN localization and previously unstudied structural features of PTEN measured through X-Ray Crystallography and Hydrogen Deuterium Exchange Mass Spectrometry. The book concludes with methods to study PTEN function in model organisms including mice and C. elegans. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, PTEN: Methods and Protocols is a valuable collection of methodologies and protocols useful to researchers who are interested in the PTEN field.


C. elegans Diabetes PTEN Hamartoma Tumor Syndrome PTEN function in brain cancer PTEN in diseased human specimens ceRNAs microRNAs protein activity protein localization

Editors and affiliations

  • Leonardo Salmena
    • 1
  • Vuk Stambolic
    • 2
  1. 1.University of TorontoPrincess Margaret Cancer CentreTorontoCanada
  2. 2.University of TorontoPrincess Margaret Cancer CentreTorontoCanada

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3297-9
  • Online ISBN 978-1-4939-3299-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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