P-Type ATPases

Methods and Protocols

  • Maike Bublitz

Part of the Methods in Molecular Biology book series (MIMB, volume 1377)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Protein Production, Isolation, Purification, and Stabilization

    1. Front Matter
      Pages 3-3
    2. Natalya U. Fedosova
      Pages 5-10
    3. Xiangyu Liu, Oleg Sitsel, Kaituo Wang, Pontus Gourdon
      Pages 29-36
    4. Hassina Azouaoui, Cédric Montigny, Aurore Jacquot, Raphaëlle Barry, Philippe Champeil, Guillaume Lenoir
      Pages 37-55
    5. Ilse Vandecaetsbeek, Peter Vangheluwe
      Pages 79-85
  3. Activity Assays

    1. Front Matter
      Pages 87-87
    2. Kathleen J. Sweadner
      Pages 89-104
    3. Pankaj Sehgal, Claus Olesen, Jesper V. Møller
      Pages 105-109
    4. Gianluca Bartolommei, Francesco Tadini-Buoninsegni
      Pages 111-120
    5. Herman G. P. Swarts, Jan B. Koenderink
      Pages 121-126
    6. Hans-Jürgen Apell, Bojana Damnjanovic
      Pages 127-156
    7. Pankaj Sehgal, Claus Olesen, Jesper V. Møller
      Pages 157-160
    8. Philip A. Bidwell, Evangelia G. Kranias
      Pages 161-170
    9. Alex Green Wielandt, Michael G. Palmgren, Anja Thoe Fuglsang, Thomas Günther-Pomorski, Bo Højen Justesen
      Pages 171-180
    10. Magdalena Marek, Thomas Günther-Pomorski
      Pages 181-191
  4. In Vitro Functional Studies

    1. Front Matter
      Pages 193-193
    2. Hiroshi Suzuki
      Pages 211-226
    3. Pankaj Sehgal, Claus Olesen, Jesper V. Møller
      Pages 227-230
  5. Ligand Binding Studies

    1. Front Matter
      Pages 231-231
    2. Johannes D. Clausen, David B. McIntosh, David G. Woolley, Jens Peter Andersen
      Pages 233-259
    3. Pankaj Sehgal, Claus Olesen, Jesper V. Møller
      Pages 261-266
    4. Teresita Padilla-Benavides, José M. Argüello
      Pages 267-277
  6. Electrophysiology

    1. Front Matter
      Pages 279-279
    2. Robert E. Dempski
      Pages 281-291
    3. Francesco Tadini-Buoninsegni, Gianluca Bartolommei
      Pages 293-303
  7. Functional Studies by Cell Culture and Transgenic Animals

    1. Front Matter
      Pages 319-319
    2. Elena Arystarkhova, Kathleen J. Sweadner
      Pages 321-332
    3. Thomas Hellesøe Holm, Toke Jost Isaksen, Karin Lykke-Hartmann
      Pages 333-340
    4. Toke Jost Isaksen, Thomas Hellesøe Holm, Karin Lykke-Hartmann
      Pages 341-351
    5. Canan Doganli, Jens Randel Nyengaard, Karin Lykke-Hartmann
      Pages 353-363
    6. Maria S. Jensen, Sara Costa, Thomas Günther-Pomorski, Rosa L. López-Marqués
      Pages 371-382
    7. Lisbeth R. Poulsen, Michael G. Palmgren, Rosa L. López-Marqués
      Pages 383-393
  8. Lipid Techniques

    1. Front Matter
      Pages 395-395
    2. Nikolaj Düring Drachmann, Claus Olesen
      Pages 397-402
    3. Jonas Lindholt Gregersen, Natalya U. Fedosova, Poul Nissen, Thomas Boesen
      Pages 403-409
  9. Crystallization

    1. Front Matter
      Pages 411-411
    2. Oleg Sitsel, Kaituo Wang, Xiangyu Liu, Pontus Gourdon
      Pages 413-420
    3. John Paul Glaves, Joseph O. Primeau, Howard S. Young
      Pages 421-441
  10. Computational Approaches in Analyzing P-Type ATPases

    1. Front Matter
      Pages 457-457
    2. Henriette Elisabeth Autzen, Maria Musgaard
      Pages 459-492

About this book


This volume provides a broad collection of protocols for many of the common experimental techniques used for the characterization of P-type ATPases.  P-Type ATPases: Methods and Protocols provides comprehensive practical instructions for all researchers in the P-type ATPase field, from the protein biochemist to the mouse geneticist, covering the identification and classification, isolation, purification, in vitro characterization, knock-out studies, as well as crystallization and structural analysis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, P-Type ATPases: Methods and Protocols aims to ensure successful results in the further study of this vital field of research.


P-type ATPases crystallization electrophysiology enzyme kinetics enzymes in vitro characterization lipid membrane membrane proteins

Editors and affiliations

  • Maike Bublitz
    • 1
  1. 1.Department of Biochemistry, BiocenterUniversity of OxfordOxfordUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3178-1
  • Online ISBN 978-1-4939-3179-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site