RNA Imaging

Methods and Protocols

  • Zdravka Medarova

Part of the Methods in Molecular Biology book series (MIMB, volume 1372)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Roger L. Kaspar, Robyn P. Hickerson, Emilio González-González, Manuel A. Flores, Tycho P. Speaker, Faye A. Rogers et al.
    Pages 1-24
  3. Marie-France Penet, Zhihang Chen, Noriko Mori, Balaji Krishnamachary, Zaver M. Bhujwalla
    Pages 37-47
  4. Steven Jones, Olivia Merkel
    Pages 79-88
  5. Muriel Golzio, Justin Teissié
    Pages 89-97
  6. Seung Koo Lee, Ching-Hsuan Tung
    Pages 113-120
  7. Jonghwan Lee, Soonhag Kim
    Pages 129-138
  8. Federico Perche, Swati Biswas, Niravkumar R. Patel, Vladimir P. Torchilin
    Pages 139-162
  9. Yoko Endo-Takahashi, Yoichi Negishi, Ryo Suzuki, Kazuo Maruyama, Yukihiko Aramaki
    Pages 209-213
  10. Back Matter
    Pages 215-218

About this book


This detailed volume will focus on the phenomenon of RNA interference by providing comprehensive coverage of various techniques for in vivo micro/siRNA imaging including the design and synthesis of specific imaging agents and tools, the development of imaging methodologies, and their interpretation. An essential element in the development and optimization of these therapies is the ability to measure the bioavailability and functionality of the RNA/oligonucleotide molecule after administration into the body. Noninvasive imaging provides the necessary set of tools to accomplish this in authentic physiologic environments and across time. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, RNA Imaging: Methods and Protocols serves physicians, scientists, and graduate students who are either new to the field of RNA-based imaging and its associated therapeutic applications or who wish to be apprised of recent advances in the state of the art.


Bioavailability MicroRNA Noninvasive imaging Post-transcriptional regulation RNA interference RNA-based imaging RNAi Therapeutic applications miRNA reprogramming siRNA

Editors and affiliations

  • Zdravka Medarova
    • 1
  1. 1.Massachusetts General HospitalCharlestownUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3147-7
  • Online ISBN 978-1-4939-3148-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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