Cytoskeleton Methods and Protocols

Methods and Protocols

  • Ray H. Gavin

Part of the Methods in Molecular Biology book series (MIMB, volume 1365)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Imaging the Cytoskeleton in Live and Fixed Cells

    1. Front Matter
      Pages 1-1
    2. Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, Liza A. Pon
      Pages 25-62
    3. Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, Liza A. Pon
      Pages 63-81
    4. Derek A. Applewhite, Christine A. Davis, Eric R. Griffis, Omar A. Quintero
      Pages 83-97
    5. Julia Dyachok, Ana Paez-Garcia, Cheol-Min Yoo, Karuppaiah Palanichelvam, Elison B. Blancaflor
      Pages 139-153
    6. Katherine Celler, Miki Fujita, Eiko Kawamura, Chris Ambrose, Klaus Herburger, Andreas Holzinger et al.
      Pages 155-184
  3. Cytoskeleton Dynamics

  4. Cell and Organelle Motility

    1. Front Matter
      Pages 263-263
    2. Deborah J. Wessels, Daniel F. Lusche, Spencer Kuhl, Amanda Scherer, Edward Voss, David R. Soll
      Pages 265-292
    3. James H.-C. Wang, Guangyi Zhao, Bin Li
      Pages 293-299
    4. James H.-C. Wang, Guangyi Zhao, Bin Li
      Pages 301-313
    5. Derrick Brazill
      Pages 339-346
  5. Genetic and Proteomic Protocols

    1. Front Matter
      Pages 347-347
    2. Michael Gotesman, Simon Menanteau-Ledouble, Mansour El-Matbouli
      Pages 357-372
    3. Michael Gotesman, Selwyn A. Williams
      Pages 373-383
    4. Michael Scarpati, Mary Ellen Heavner, Eliza Wiech, Shaneen Singh
      Pages 385-413
    5. Giovanni J. Pagano, Linda A. Hufnagel, Roberta S. King
      Pages 415-427
  6. Back Matter
    Pages 429-431

About this book


The third edition of this volume focuses on experimental models that are useful for investigating various aspects of cytoskeleton structure and function. Animal, plant, protist, and fungal models highlight twenty-four chapters that provide detailed protocols for live and fixed-cell imaging, dynamics of cytoskeleton components, cell and organelle motility, and genetics and proteomics. Written in the highly successful Methods in Molecular Biology series format, protocols in each chapter are up-to-date menus organized in a useful step-by-step format appropriate for novice and established investigators. Each chapter is equipped with a valuable notes section that provides a troubleshooting guide and helpful, and often unpublished, technical information aimed at ensuring success with implementation of the protocols.

Authoritative and thorough, Cytoskeleton Methods and Protocols, Third Edition helps researchers expand their understanding of cytoskeleton structure and function.


Cell and Organelle Motility Cell-Imaging Cytoskeleton Components Cytoskeleton Function Cytoskeleton Structure In Vitro Reconstructions Proteomics

Editors and affiliations

  • Ray H. Gavin
    • 1
  1. 1.Brooklyn CollegeBrooklynUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3123-1
  • Online ISBN 978-1-4939-3124-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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