Receptor and Ion Channel Detection in the Brain

Methods and Protocols

  • Rafael Luján
  • Francisco Ciruela

Part of the Neuromethods book series (NM, volume 110)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Molecular Techniques

    1. Front Matter
      Pages 1-1
    2. Xavier Morató, Dasiel O. Borroto-Escuela, Kjell Fuxe, Víctor Fernández-Dueñas, Francisco Ciruela
      Pages 19-29
    3. Paula M. Canas, Rodrigo A. Cunha
      Pages 31-37
    4. Juan Varela, Julien Dupuis, Laurent Groc
      Pages 49-58
    5. Fuencisla Pilar-Cuéllar, Alvaro Díaz, Emilio Garro-Martínez, Alicia Martín, Beatriz Romero, Elsa M. Valdizán
      Pages 59-76
    6. Víctor Fernández-Dueñas, Thierry Durroux, Francisco Ciruela
      Pages 99-107
    7. Dasiel O. Borroto-Escuela, Beth Hagman, Miles Woolfenden, Luca Pinton, Antonio Jiménez-Beristain, Julia Oflijan et al.
      Pages 109-124
  3. Neuroanatomical Techniques

    1. Front Matter
      Pages 125-125
    2. Miwako Yamasaki, Masahiko Watanabe
      Pages 127-142
    3. Rebeca Vidal, Raquel Linge, María Josefa Castillo, Josep Amigó, Elsa M. Valdizán, Elena Castro
      Pages 143-156
    4. Sara Gil-Perotin, A. Cebrián-Silla, V. Herranz-Pérez, P. García-Belda, S. Gil-García, M. Fil et al.
      Pages 179-190
    5. Hiroyuki Hioki, Hisashi Nakamura, Takahiro Furuta
      Pages 247-266
    6. Takahiro Furuta, Keiko Okamoto-Furuta, Hiroyuki Hioki
      Pages 267-275
  4. Functional Techniques

    1. Front Matter
      Pages 311-311
    2. Agnès Gruart, José M. Delgado-García
      Pages 369-396
    3. Kendra D. Bunner, George V. Rebec
      Pages 397-414
    4. Jorge E. Ortega, J. Javier Meana, Luis F. Callado
      Pages 415-434
    5. Rhiannon M. Meredith, Martine R. Groen
      Pages 435-445
    6. Víctor Fernández-Dueñas, Xavier Morató, Thomas Knöpfel, Francisco Ciruela
      Pages 447-454
    7. Jaume Taura, Víctor Fernández-Dueñas, Francisco Ciruela
      Pages 455-463
    8. Maricel Gómez-Soler, Víctor Fernández-Dueñas, Francisco Ciruela
      Pages 465-472
  5. Back Matter
    Pages 473-475

About this book


Receptor and Ion Channel Detection in the Brain provides state-of-the-art and up-to-date methodological information on molecular, neuroanatomical and functional techniques that are currently used to study neurotransmitter receptors and ion channels in the brain. The chapters have been contributed by world-wide recognized neuroscientists who explain in an easy and detailed way well established and tested protocols embracing molecular, cellular, subcellular, anatomical and electrophysiological aspects of the brain. This comprehensive and practical manual is presented in a simple, step-by-step manner for laboratory use, and also offers unambiguous detail and key implementation advice that proves essential for successful results and facilitate choosing the best method for the target proteins under study. This work serves as a useful guide for young researchers and students in training as well as for neurologists and established scientists who wish to extend their repertoire of techniques.


Biochemical approaches Electrophysiological recordings Fluorescence & FRET assays Neuroanatomical techniques Neurochemical determinations

Editors and affiliations

  • Rafael Luján
    • 1
  • Francisco Ciruela
    • 2
  1. 1.Research Institute on Neurological Disabilities (IDINE), Department of Medical Sciences, School of MedicineUniversity of Castilla- La ManchaAlbaceteSpain
  2. 2.Pharmacology Unit, Department of Pathology and Experimental Therapeutics, School of Medicine, IDIBELLUniversity of BarcelonaBarcelonaSpain

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3063-0
  • Online ISBN 978-1-4939-3064-7
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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