In Vitro Embryogenesis in Higher Plants

  • Maria Antonietta Germana
  • Maurizio Lambardi

Part of the Methods in Molecular Biology book series (MIMB, volume 1359)

Table of contents

  1. Front Matter
    Pages i-xviii
  2. Reviews on General Topics

    1. Front Matter
      Pages 1-1
    2. Melanie L. Hand, Sacco de Vries, Anna M. G. Koltunow
      Pages 3-23
    3. Maria Maddalena Altamura, Federica Della Rovere, Laura Fattorini, Simone D’Angeli, Giuseppina Falasca
      Pages 47-85
    4. Birgit Arnholdt-Schmitt, Carla Ragonezi, Hélia Cardoso
      Pages 87-100
    5. Shuanglong Huang, Mohamed M. Mira, Claudio Stasolla
      Pages 101-115
    6. Sandra I. Correia, Ana C. Alves, Paula Veríssimo, Jorge M. Canhoto
      Pages 117-129
    7. Krystyna Klimaszewska, Catherine Hargreaves, Marie-Anne Lelu-Walter, Jean-François Trontin
      Pages 131-166
    8. Jean-François Trontin, Krystyna Klimaszewska, Alexandre Morel, Catherine Hargreaves, Marie-Anne Lelu-Walter
      Pages 167-207
    9. Jose M. Seguí-Simarro
      Pages 209-244
    10. Liwen Fei, Pamela Weathers
      Pages 245-259
  3. Protocols of Somatic Embryogenesis in Selected Important Horticultural Plants

    1. Front Matter
      Pages 261-261
    2. Sadanand A. Dhekney, Zhijian T. Li, Trudi N. L. Grant, Dennis J. Gray
      Pages 263-277
    3. Douglas A. Steinmacher, Angelo Schuabb Heringer, Víctor M. Jiménez, Marguerite G. G. Quoirin, Miguel P. Guerra
      Pages 279-288
    4. Ahmad A. Omar, Manjul Dutt, Frederick G. Gmitter, Jude W. Grosser
      Pages 289-327
    5. João F. Martins, Sandra I. Correia, Jorge M. Canhoto
      Pages 329-339
    6. Basar Sevindik, Yesim Yalcin Mendi
      Pages 351-357
    7. Barbara Ruffoni, Laura Bassolino
      Pages 359-370
    8. Jaime A. Teixeira da Silva, Budi Winarto
      Pages 371-386
    9. Glaucia B. Cabral, Vera T. C. Carneiro, Diva M. A. Dusi, Adriana P. Martinelli
      Pages 395-402
  4. Protocols of Somatic Embryogenesis in Selected Forest Trees

    1. Front Matter
      Pages 403-403
    2. Itziar Aurora Montalbán, Olatz García-Mendiguren, Paloma Moncaleán
      Pages 405-415
    3. Jana Krajňáková, Hely Häggman
      Pages 417-430
    4. Miguel P. Guerra, Neusa Steiner, Francine L. Farias-Soares, Leila do N. Vieira, Hugo P. F. Fraga, Gladys D. Rogge-Renner et al.
      Pages 439-450
  5. Protocols of Gametic Embryogenesis in Selected Higher Plants

    1. Front Matter
      Pages 451-451
    2. Giuseppe Leonardo Rotino
      Pages 453-466
    3. Verónica Parra-Vega, Jose M. Seguí-Simarro
      Pages 467-474
    4. Benedetta Chiancone, Maria Antonietta Germanà
      Pages 475-487
  6. Stepwise Protocols on Pivotal Topics

  7. Back Matter
    Pages 551-558

About this book


This volume presents an overview of recent advances, innovative applications, and future prospects of in vitro embryogenesis in higher plants. The book's chapters are divided into five parts: Part I contains reviews on general topics (microspore; zygotic and somatic embryogenesis; in vitro and in vivo asexual embryogenesis; advances on the genetic, physiological, and proteomic knowledge of somatic embryo formation; role of apoptosis and mitochondria in somatic embryogenesis; and innovation in the use of bioreactors). The remaining four parts discuss step-wise protocols on somatic embryogenesis in selected horticultural plants (Part II); forest trees (Part III); gametic embryogenesis (Part IV); and pivotal topics, such as the detection of epigenetics modifications during microspore embryogenesis, the in vitro embryogenesis and plant regenerations from isolated zygotes, the synthetics seed production, the induction and maturation of somatic embryos, and the cryostorage of embryogenic cultures (Part V). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and thorough, In Vitro Embryogenesis in Higher Plants, is a useful source of information and ideas for plant tissue culturists, cell biologists, embryologists, horticulturists, and operators of commercial nurseries. This book will introduce the fascinating work in in vitro embryogenesis in higher plants to students and young scientists.


Embryogenesis Gametic Horticulture In Vitro Embryo Induction In Vivo Asexual Embryogenesis Microspore Plant Regeneration Somatic Zygotic

Editors and affiliations

  • Maria Antonietta Germana
    • 1
  • Maurizio Lambardi
    • 2
  1. 1.Dipartimento DEMETRAUniversità degli Studi di PalermoPalermoItaly
  2. 2.National Research Council (CNR) IVALSA/Istituto per la ValorizzazioneSesto Fiorentino, FlorenceItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3060-9
  • Online ISBN 978-1-4939-3061-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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