Methods and Protocols

  • Louise von Stechow

Part of the Methods in Molecular Biology book series (MIMB, volume 1355)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Techniques for Tagging Phospho-Moieties

    1. Front Matter
      Pages 1-1
    2. Emiko Kinoshita-Kikuta, Eiji Kinoshita, Tohru Koike
      Pages 17-29
  3. Labeling Techniques for Quantitative Phosphoproteomics

    1. Front Matter
      Pages 51-51
    2. Lei Cheng, Trairak Pisitkun, Mark A. Knepper, Jason D. Hoffert
      Pages 53-70
    3. Daniel A. Rothenberg, Elizabeth A. Gordon, Forest M. White, Sebastian Lourido
      Pages 71-84
  4. Phosphopeptide Enrichment Strategies

  5. Phosphoprotein Analysis by Mass Spectrometry

    1. Front Matter
      Pages 211-211
    2. Humberto Gonczarowska-Jorge, Margherita Dell’Aica, Clarissa Dickhut, René P. Zahedi
      Pages 225-239
    3. Hongyan Zhao, Andrew J. Creese, Helen J. Cooper
      Pages 241-250
    4. Rosa R. Jersie-Christensen, Abida Sultan, Jesper V. Olsen
      Pages 251-260
  6. High-Throughput Studies of the Phosphoproteome

    1. Front Matter
      Pages 261-261
    2. Liang Xue, Justine V. Arrington, W. Andy Tao
      Pages 263-273
    3. Johannes Landskron, Kjetil Taskén
      Pages 275-290
  7. Bioinformatics Resources for Phosphoproteomics Analyses

    1. Front Matter
      Pages 291-291
    2. Vaishnavi Ravikumar, Boris Macek, Ivan Mijakovic
      Pages 293-306
    3. Stephanie Munk, Jan C. Refsgaard, Jesper V. Olsen, Lars J. Jensen
      Pages 307-321
    4. Jan C. Refsgaard, Stephanie Munk, Lars J. Jensen
      Pages 323-339
    5. Stephanie Munk, Jan C. Refsgaard, Jesper V. Olsen
      Pages 341-360
  8. Back Matter
    Pages 361-364

About this book


This second edition provides up-to-date protocols focusing on techniques for the specific enrichment of phosphopeptides and phosphoproteins. Phospho-Proteomics Methods and Protocols, Second Edition guides readers through different labeling strategies for quantitative phosphoproteomics; high-throughput mass spectrometry-based phosphoproteome analyses and phospho flow cytometry; and bioinformatics strategies for phosphoproteomics data analysis and integration. Additional protocols concentrate on the identification of kinase-substrate relationships by both high- and low-throughput approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Phospho-Proteomics Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.


Ser residues Thr residues Tyr residues kinase-substrate relationships phospho flow cytometry phosphopeptides phosphoproteins phosphorylated proteins quantitative phosphoproteomics tissue samples

Editors and affiliations

  • Louise von Stechow
    • 1
  1. 1.University of CopenhagenCenter for Protein ResearchCopenhagen NmDenmark

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3048-7
  • Online ISBN 978-1-4939-3049-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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