HIV Protocols

  • Vinayaka R. Prasad
  • Ganjam V. Kalpana

Part of the Methods in Molecular Biology book series (MIMB, volume 1354)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. HIV-1 Replication—Early Events

  3. HIV-1 RNA Structure and RNA-Protein Interactions

    1. Front Matter
      Pages 89-89
    2. Jason W. Rausch, Joanna Sztuba-Solinska, Sabrina Lusvarghi, Stuart F. J. Le Grice
      Pages 91-117
    3. Sebla B. Kutluay, Paul D. Bieniasz
      Pages 119-131
  4. HIV-1 Replication—Post-integration Events

    1. Front Matter
      Pages 147-147
    2. Jacques J. Kessl, Amit Sharma, Mamuka Kvaratskhelia
      Pages 149-164
    3. Annalena La Porte, Ganjam V. Kalpana
      Pages 165-174
  5. Studying HIV-1 Replication and Pathogenesis in Animal Models

    1. Front Matter
      Pages 187-187
    2. Radwa Sharaf, Thorsten R. Mempel, Thomas T. Murooka
      Pages 189-201
    3. Milena Veselinovic, Paige Charlins, Ramesh Akkina
      Pages 203-220
    4. Tynisha Thomas, Kieran Seay, Jian Hua Zheng, Cong Zhang, Christina Ochsenbauer, John C. Kappes et al.
      Pages 221-235
  6. Tools to Study HIV-1 Latency and Pathogenesis

    1. Front Matter
      Pages 237-237
    2. Gregory M. Laird, Daniel I. S. Rosenbloom, Jun Lai, Robert F. Siliciano, Janet D. Siliciano
      Pages 239-253
    3. Ulrike Jung, Mayumi Takahashi, John J. Rossi, John C. Burnett
      Pages 255-264
    4. Nigora Mukhamedova, Beda Brichacek, Christina Darwish, Anastas Popratiloff, Dmitri Sviridov, Michael Bukrinsky
      Pages 281-292
    5. Nicole A. Haverland, Lance M. Villeneuve, Pawel Ciborowski, Howard S. Fox
      Pages 293-310
  7. NeuroAIDS

    1. Front Matter
      Pages 327-327
    2. Fabienne Rayne, Solène Debaisieux, Annie Tu, Christophe Chopard, Petra Tryoen-Toth, Bruno Beaumelle
      Pages 329-342
    3. Vasudev R. Rao, Eliseo A. Eugenin, Vinayaka R. Prasad
      Pages 367-376
  8. Fabienne Rayne, Solène Debaisieux, Annie Tu, Christophe Chopard, Petra Tryoen-Toth, Bruno Beaumelle
    Pages E1-E1
  9. Back Matter
    Pages 377-383

About this book


This third edition volume expands upon the previous editions with new research topics and numerous leading-edge HIV protocols. This book contains chapters that cover various aspects of HIV-1 replication and are helpful for investigating the mechanisms of HIV-1 replication or pathogenesis. The chapters are grouped into six sections: HIV early events; HIV-1 RNA structure and RNA-protein interactions; HIV-1 post-integration events; HIV-1 pathogenesis in animal models; tools to study HIV-1 latency; and NeuroAIDS. Chapters are written by research experts who have diligently developed techniques, improved them, simplified them, or applied them to their own HIV research. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, HIV Protocols, Third Edition is a great resource for HIV research, and contains many significant tips and tricks rarely mentioned in standard protocols.


HIV-1 RNA structure HIV-1 latency HIV-1 replication NeuroAIDS RNA protein interactions Virology

Editors and affiliations

  • Vinayaka R. Prasad
    • 1
  • Ganjam V. Kalpana
    • 2
  1. 1.Department of Microbiology & ImmunologyAlbert Einstein College of MedicineBRONXUSA
  2. 2.Department of GeneticsAlbert Einstein College of MedicineBRONXUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-3045-6
  • Online ISBN 978-1-4939-3046-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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