Peptide Antibodies

Methods and Protocols

  • Gunnar Houen

Part of the Methods in Molecular Biology book series (MIMB, volume 1348)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Hui Ma, Richard O’Kennedy
    Pages 7-11
  3. Claudio Peri, Oscar C. Solé, Dario Corrada, Alessandro Gori, Xavier Daura, Giorgio Colombo
    Pages 13-22
  4. Morten Nielsen, Paolo Marcatili
    Pages 23-32
  5. Paul R. Hansen, Alberto Oddo
    Pages 33-50
  6. Paul R. Hansen
    Pages 51-57
  7. Gunnar Houen, Dorthe T. Olsen
    Pages 59-64
  8. Bala K. Prabhala, Osman Mirza, Peter Højrup, Paul R. Hansen
    Pages 77-82
  9. Tina H. Pihl, Kristin E. Illigen, Gunnar Houen
    Pages 103-107
  10. Nicole Hartwig Trier, Anne Mortensen, Annette Schiolborg, Tina Friis
    Pages 109-126
  11. Angelo Bolchi, Elena Canali, Andrea Santoni, Gloria Spagnoli, Daniele Viarisio, Rosita Accardi et al.
    Pages 137-151
  12. Norah A. Alturki, Kevin A. Henry, C. Roger MacKenzie, Mehdi Arbabi-Ghahroudi
    Pages 167-189
  13. Brian H. Santich, Hong Liu, Cheng Liu, Nai-Kong V. Cheung
    Pages 191-204
  14. Anna Chailyan, Paolo Marcatili
    Pages 205-214
  15. Angela Filomena, Yvonne Beiter, Markus F. Templin, Thomas O. Joos, Nicole Schneiderhan-Marra, Oliver Poetz
    Pages 251-265
  16. Feliciana Real-Fernández, Giada Rossi, Filomena Panza, Federico Pratesi, Paola Migliorini, Paolo Rovero
    Pages 267-274
  17. Goran Kungulovski, Ina Kycia, Rebekka Mauser, Albert Jeltsch
    Pages 275-284
  18. Peter M. H. Heegaard, Ann-Louise Bergström, Heidi Gertz Andersen, Henriette Cordes
    Pages 285-301
  19. Tina Friis, Klaus Boberg Pedersen, David Hougaard, Gunnar Houen
    Pages 311-325
  20. Harinder Singh, Sudheer Gupta, Ankur Gautam, Gajendra P. S. Raghava
    Pages 327-340
  21. Chit Laa Poh, Katherine Kirk, Hui Na Chua, Lara Grollo
    Pages 341-350
  22. Anders Fomsgaard
    Pages 351-357
  23. Back Matter
    Pages 359-366

About this book


This extensive volume covers basic and advanced aspects of peptide antibody production, characterization, and uses. Although peptide antibodies have been available for many years, they continue to be a field of active research and method development. For example, peptide antibodies which are dependent on specific posttranslational modifications are of great interest, such as phosphorylation, citrullination, and others, while different forms of recombinant peptide antibodies are gaining interest, notably nanobodies, single chain antibodies, TCR-like antibodies, among others. Within this volume, those areas are covered, as well as several technical and scientific advances: solid phase peptide synthesis, peptide carrier conjugation and immunization, genomics, transcriptomics, proteomics and elucidation of the molecular basis of antigen presentation and recognition by dendritic cells, macrophages, B cells and T cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Comprehensive and authoritative, Peptide Antibodies: Methods and Protocols serves as an ideal reference for researchers exploring this vital and expansive area of study.


Antibody characterization Immunology Peptide antibody production Post-translational modifications Recombinant peptide antibodies

Editors and affiliations

  • Gunnar Houen
    • 1
  1. 1.Statens Serum InstitutCopenhagenDenmark

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2998-6
  • Online ISBN 978-1-4939-2999-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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