TGF-β Signaling

Methods and Protocols

  • Xin-Hua Feng
  • Pinglong Xu
  • Xia Lin

Part of the Methods in Molecular Biology book series (MIMB, volume 1344)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Erine H. Budi, Jian Xu, Rik Derynck
    Pages 1-33
  3. Long Zhang, Fangfang Zhou, Maarten van Dinther, Peter ten Dijke
    Pages 35-47
  4. Xiaohua Yan, Ye-Guang Chen
    Pages 49-61
  5. Fang Liu, Isao Matsuura
    Pages 93-109
  6. Tao Shen, Lan Qin, Xia Lin
    Pages 111-119
  7. Wenlin Li, Wanguo Wei, Sheng Ding
    Pages 137-145
  8. Ulrich Valcourt, Jonathon Carthy, Yukari Okita, Lindsay Alcaraz, Mitsuyasu Kato, Sylvie Thuault et al.
    Pages 147-181
  9. Takashi Sekiya, Akihiko Yoshimura
    Pages 183-191
  10. J. A. Maring, L. A. van Meeteren, M. J. Goumans, Peter ten Dijke
    Pages 193-203
  11. Maria Namwanje, Juan C. Bournat, Chester W. Brown
    Pages 205-217
  12. Marco Montagner, Graziano Martello, Stefano Piccolo
    Pages 245-259
  13. Chenbei Chang
    Pages 261-274
  14. Xingfeng Liu, Qiang Wang, Anming Meng
    Pages 275-286
  15. Janet L. Crane, Lingling Xian, Xu Cao
    Pages 287-300
  16. Chao Yu, Jian-Jie Zhou, Heng-Yu Fan
    Pages 301-311
  17. Pengyuan Yang, Yun Zhang, Geoffrey J. Markowitz, Xing Guo, Xiao-Fan Wang
    Pages 325-339
  18. Atsushi Kaneda, Aya Nonaka, Takanori Fujita, Ryota Yamanaka, Mai Fujimoto, Kohei Miyazono et al.
    Pages 341-353
  19. Shyam Kumar Gudey, Marene Landström
    Pages 355-363
  20. Zipei Feng, Zhike Zi, Xuedong Liu
    Pages 379-389
  21. Back Matter
    Pages 391-394

About this book


This volume details up-to-date information and attempts to take the reader into the exciting realm of TGF-β from the basic principles to the practical applications. Chapters provide basic introduction of TGF-β signaling from the cell surface to the nucleus, methods and techniques for the investigation of TGF-β signaling mechanism including receptors, Smads, intracellular kinases, microRNA, epigenetic regulation, post-translational regulations, non-Smad pathway; the physiological implications including those in cell cycle arrest, epithelial-mesenchymal transition, endothelial cells, adipogenesis, Th differentiation, stem cell, bone remodeling, ovary, zebrofish development, and frog animal capping; and the methodologies including metastasis imaging, 3D morphogenesis, membrane receptor quantification, conditional knockout, bone remodeling, kinase and phosphatase assays, BiFC interaction assays, and genome-wide siRNA screen. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, TGF-Beta Signaling: Methods and Protocols aims to ensure successful results in the further study of this vital field.


Th differentiation adipogenesis bone remodeling cell surface epigenetic regulation epithelial-mesenchymal transition intracellular kinases microRNA non-Smad pathway post-translational regulations

Editors and affiliations

  • Xin-Hua Feng
    • 1
  • Pinglong Xu
    • 2
  • Xia Lin
    • 3
  1. 1.Departments of Surgery and Molecular & Cellular BiologyBaylor College of MedicineHoustonUSA
  2. 2.Life Sciences InstituteZhejiang UniversityHangzhou, Zhejiang ProviinceChina
  3. 3.Department of SurgeryBaylor College of MedicineHoustonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2965-8
  • Online ISBN 978-1-4939-2966-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals