Cartilage Tissue Engineering

Methods and Protocols

  • Pauline M. Doran

Part of the Methods in Molecular Biology book series (MIMB, volume 1340)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Overview

    1. Front Matter
      Pages 1-1
  3. Cell Isolation, Expansion, and Differentiation

    1. Front Matter
      Pages 23-23
    2. Kifah Shahin, Nastaran Mahmoudifar, Pauline M. Doran
      Pages 25-40
    3. Oliver F. W. Gardner, Mauro Alini, Martin J. Stoddart
      Pages 41-52
    4. Nastaran Mahmoudifar, Pauline M. Doran
      Pages 53-64
    5. Hicham Drissi, Jason D. Gibson, Rosa M. Guzzo, Ren-He Xu
      Pages 65-78
  4. Biomaterials and Scaffolds

    1. Front Matter
      Pages 119-119
    2. Peggy P. Y. Chan
      Pages 121-132
    3. Shraddha Thakkar, Hugo Fernandes, Lorenzo Moroni
      Pages 133-151
    4. Ting Ting Lau, Wenyan Leong, Yvonne Peck, Kai Su, Dong-An Wang
      Pages 153-160
    5. Hyun-Wook Kang, James J. Yoo, Anthony Atala
      Pages 161-169
    6. Marie Morille, Marie-Claire Venier-Julienne, Claudia N. Montero-Menei
      Pages 171-180
    7. Clara R. Correia, Rui L. Reis, João F. Mano
      Pages 181-189
    8. Patcharakamon Nooeaid, Gundula Schulze-Tanzil, Aldo R. Boccaccini
      Pages 191-200
  5. Bioreactors

    1. Front Matter
      Pages 201-201
    2. Joanna F. Weber, Roman Perez, Stephen D. Waldman
      Pages 203-219
    3. Kifah Shahin, Pauline M. Doran
      Pages 221-233
    4. Yu-Han Chang, Min-Hsien Wu
      Pages 235-244
  6. In Vivo Application

    1. Front Matter
      Pages 245-245
  7. Evaluation

    1. Front Matter
      Pages 261-261
    2. Xinzhu Pu, Julia Thom Oxford
      Pages 263-278
    3. Dinorath Olvera, Andrew Daly, Daniel John Kelly
      Pages 279-287
  8. Back Matter
    Pages 289-290

About this book


This volume aims to describe clearly and in detail the key practical skills involved in cartilage tissue engineering. Methods are outlined for isolation and expansion of chondrocytes and stem cells; differentiation; synthesis and application of three-dimensional scaffolds; design and operation of bioreactors; in vivo testing of engineered constructs; and molecular and functional analysis of cartilage cells and tissues. Frequently used technologies are covered, as well as more recent advances in inspirational areas such as ‘smart’ biomaterial development, novel bioreactor design, -omics analysis, and genetic manipulation of matrix synthesis. The key procedures discussed either underpin the progress already achieved in cartilage tissue engineering or are indicative of the direction of future research in the area. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, readily reproducible step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cartilage Tissue Engineering: Methods and Protocols is a useful resource that informs the scientific community about the experimental work covering a broad range of objectives for cartilage synthesis and regeneration. 


Bioreactors Cartilage Cellular Differentiation Chondrogenic Cells Stem Cells Synthesis Tissue Engineering

Editors and affiliations

  • Pauline M. Doran
    • 1
  1. 1.Swinburne University of TechnologyFaculty of Science, Engineering and TechnologyHawthorn, MelbourneAustralia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2937-5
  • Online ISBN 978-1-4939-2938-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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