Methods and Protocols

  • Ralf Kühn
  • Wolfgang Wurst
  • Benedikt Wefers

Part of the Methods in Molecular Biology book series (MIMB, volume 1338)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Annekatrin Richter, Jana Streubel, Jens Boch
    Pages 9-25
  3. David G. Ousterout, Charles A. Gersbach
    Pages 27-42
  4. Adam J. Bogdanove, Nicholas J. Booher
    Pages 43-47
  5. Junjiao Yang, Shengjie Guo, Pengfei Yuan, Wensheng Wei
    Pages 49-60
  6. Tetsushi Sakuma, Takashi Yamamoto
    Pages 61-70
  7. Marion Piganeau, Benjamin Renouf, Hind Ghezraoui, Erika Brunet
    Pages 99-117
  8. Toshinori Hayashi, Takashi Takeuchi
    Pages 119-126
  9. Yoko Takasu, Toshiki Tamura, Marian Goldsmith, Michal Zurovec
    Pages 127-142
  10. Sanjay Basu, Azadeh Aryan, Mary Etna Haac, Kevin M. Myles, Zach N. Adelman
    Pages 157-177
  11. Jiyong Liu, Yawen Guo, Changqing Li, Yixu Chen, Renjie Jiao
    Pages 179-190
  12. Peng Huang, An Xiao, Xiangjun Tong, Shuo Lin, Bo Zhang
    Pages 191-206
  13. Yun Liu, Hui Zhao, Christopher H. K. Cheng
    Pages 207-227
  14. Benedikt Wefers, Christina Brandl, Oskar Ortiz, Wolfgang Wurst, Ralf Kühn
    Pages 229-243
  15. Laurent Tesson, Séverine Remy, Séverine Ménoret, Claire Usal, Reynald Thinard, Chloé Savignard et al.
    Pages 245-259
  16. Tobias Schmidt, Jonathan L. Schmid-Burgk, Thomas S. Ebert, Moritz M. Gaidt, Veit Hornung
    Pages 261-272
  17. Back Matter
    Pages 273-274

About this book


This volume provides a comprehensive collection of protocols on new technology across various model organisms.  Chapters describe species-specific methods to generate new mutants the content is completed by chapters on natural TAL effectors, TAL element DNA binding principles, TALEN target site prediction, and methods for the efficient construction of TALEN coding regions. While addition chapter focus on the application of TALEN as sequence-specific nucleases and TAL based gene activators or inhibitors and the visualization of chromatin dynamics in live cells.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, TALENs: Methods and Protocols aims to ensure successful results in the further study of this vital field.


DNA sequence recognition TAL based gene activators TAL element DNA binding principles TALEN coding regions TALEN target site prediction chromatin dynamics in live cells natural TAL effectors nuclease technology sequence-specific nucleases short RNAs

Editors and affiliations

  • Ralf Kühn
    • 1
  • Wolfgang Wurst
    • 2
  • Benedikt Wefers
    • 3
  1. 1.Max-Delbrück-Center for Molecular MedicineBerlinGermany
  2. 2.Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental HealthNeuherbergGermany
  3. 3.Institute of Developmental Genetics, Helmholtz Zentrum München, German Research Center for Environmental HealthNeuherbergGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2931-3
  • Online ISBN 978-1-4939-2932-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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