Bacterial Persistence

Methods and Protocols

  • Jan Michiels
  • Maarten Fauvart

Part of the Methods in Molecular Biology book series (MIMB, volume 1333)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Natalie Verstraeten, Wouter Knapen, Maarten Fauvart, Jan Michiels
      Pages 3-13
  3. Quantification of Persistence

    1. Front Matter
      Pages 15-15
    2. Sarah E. Rowe, Brian P. Conlon, Iris Keren, Kim Lewis
      Pages 17-28
    3. Niilo Kaldalu, Arvi Jõers, Henri Ingelman, Tanel Tenson
      Pages 29-42
    4. Ilse Vandecandelaere, Heleen Van Acker, Tom Coenye
      Pages 53-66
    5. Katrijn De Brucker, Kaat De Cremer, Bruno P. A. Cammue, Karin Thevissen
      Pages 67-72
  4. Single Cell Analysis of Persister Cells

    1. Front Matter
      Pages 73-73
    2. Irit Levin-Reisman, Nathalie Q. Balaban
      Pages 75-81
    3. Mehmet A. Orman, Theresa C. Henry, Christina J. DeCoste, Mark P. Brynildsen
      Pages 83-100
    4. Ryota Iino, Shouichi Sakakihara, Yoshimi Matsumoto, Kunihiko Nishino
      Pages 101-109
  5. Identification of Persister Mutants and Genes

    1. Front Matter
      Pages 111-111
    2. Bram Van den Bergh, Joran E. Michiels, Jan Michiels
      Pages 131-143
  6. Cellular and Animal Model Systems for Studying Persistence

    1. Front Matter
      Pages 145-145
    2. Julien M. Buyck, Sandrine Lemaire, Cristina Seral, Ahalieyah Anantharajah, Frédéric Peyrusson, Paul M. Tulkens et al.
      Pages 147-157
    3. Thomas J. Hannan, David A. Hunstad
      Pages 159-175

About this book

Introduction

This volume presents a comprehensive collection of methods that have been instrumental to the current understanding of bacterial persisters. Chapters in the book cover topics ranging from general methods for measuring persister levels in Escherichia coli cultures, protocols for the determination of the persister subpopulation in Candida albicans, quantitative measurements of Type I and Type II persisters using ScanLag, to in vitro and in vivo models for the study of the intracellular activity of antibiotics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols brings together the most respected researchers in bacterial persistence whose studies will remain vital to understanding this field for many years to come. 

Keywords

Antibiotics Bacteria Mycobacterium Tuberculosis Persister Cells Pseudomonas Aeruginosa Staphylococcus Aureus

Editors and affiliations

  • Jan Michiels
    • 1
  • Maarten Fauvart
    • 2
  1. 1.Department of Microbial and Molecular SystemsKU Leuven - University of LeuvenHeverleeBelgium
  2. 2.Department of Microbial and Molecular SystemsKU Leuven - University of LeuvenHeverleeBelgium

Bibliographic information

  • DOI https://doi.org/10.1007/978-1-4939-2854-5
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2853-8
  • Online ISBN 978-1-4939-2854-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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