Hedgehog Signaling Protocols

  • Natalia A. Riobo

Part of the Methods in Molecular Biology book series (MIMB, volume 1322)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Irene Seijo-Barandiarán, Isabel Guerrero, Marcus Bischoff
    Pages 9-18
  3. Óscar Sánchez, Juan Calvo, Carmen Ibáñez, Isabel Guerrero, Juan Soler
    Pages 19-33
  4. Shohreh F. Farzan, David J. Robbins
    Pages 93-104
  5. Robert Ahrends, Pawel Niewiadomski, Mary N. Teruel, Rajat Rohatgi
    Pages 105-123
  6. Pawel Niewiadomski, Rajat Rohatgi
    Pages 125-130
  7. Paola Infante, Romina Alfonsi, Lucia Di Marcotullio
    Pages 131-146
  8. Sonia Coni, Laura Di Magno, Gianluca Canettieri
    Pages 147-156
  9. João Francisco Botelho, Daniel Smith-Paredes, Verónica Palma A.
    Pages 157-166
  10. Donna M. Brennan-Crispi, Mỹ G. Mahoney, Natalia A. Riobo
    Pages 167-185
  11. Back Matter
    Pages 199-200

About this book


The second edition of this volume provides novel protocols for the study of newly discovered function and modifications of components of the Hh pathways. The first few chapters discuss methods for stable delivery of soluble Hh proteins in cell cultures that can be adapted for in vivo studies, and mathematical models for studying Hh proteins gradient formation in tissues. The next few chapters explore studies in cell culture using fibroblasts that respond readily to Hh proteins. The book goes on to present protocols for evaluation of transcriptional activity of the Gli transcription factors in cell cultures and determine the expression level of endogenous Gli proteins. The final chapters talk about metabolic effects of noncanonical Hh signaling. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, Hedgehog Signaling Protocols, Second Edition presents researchers with a comprehensive understanding of the Hh pathway and its function in development and diseases.


Cell Culture Cytonemes Drosophila Fibroblasts G Protein-Coupled Receptor Gli Proteins Hedgehog (Hh) Proteins Hh Pathway Noncanonical Hh Signaling

Editors and affiliations

  • Natalia A. Riobo
    • 1
  1. 1.Department of Biochemistry and Molecular Biology, Sidney Kimmel Cancer CenterThomas Jefferson UniversityPhiladelphiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2771-5
  • Online ISBN 978-1-4939-2772-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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