Epigenetic Methods in Neuroscience Research

  • Nina Karpova

Part of the Neuromethods book series (NM, volume 105)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Francesco Matrisciano, Isabella Panaccione, Erbo Dong, Dennis R. Grayson, Alessandro Guidotti
      Pages 3-18
  3. Analysis of Chromatin Remodeling

    1. Front Matter
      Pages 53-53
    2. Nina N. Karpova, Juzoh Umemori
      Pages 97-114
    3. Miki Bundo, Tadafumi Kato, Kazuya Iwamoto
      Pages 115-123
    4. Koji Hayakawa, Chikako Yoneda, Ruiko Tani, Kunio Shiota
      Pages 139-147
    5. Amanda Sales, Caroline Biojone, Sâmia Joca
      Pages 149-159
  4. Analysis of Non-coding Genome

    1. Front Matter
      Pages 161-161
    2. Brian B. Griffiths, Richard G. Hunter
      Pages 163-174
    3. Asli Silahtaroglu, Silke Herzer, Björn Meister
      Pages 195-208
    4. Sharon L. Hollins, Fredrick R. Walker, Murray J. Cairns
      Pages 209-241
  5. Analysis of Circadian Oscilations

    1. Front Matter
      Pages 243-243
    2. Hitoshi Iuchi, Rikuhiro G. Yamada, Hiroki R. Ueda
      Pages 245-254
  6. Back Matter
    Pages 255-258

About this book


This volume presents state-of-the-art methods for reliable detection of epigenetic changes in the nervous system. Epigenetic Methods in Neuroscience Research  guides readers through methods for the analyses of chromatin remodeling, transposable elements, non-coding RNAs, such as miRNAs, and circadian oscillations, including: analysis of DNA methylation in neuronal and glial cells or small tissue samples; sensitive method for quantification of alternative methylated forms of cytosines by liquid chromatography/mass spectrometry; affinity-based detection of modified cytosines by immunohistochemistry or methylated DNA immunoprecipitation; chromatin immunoprecipitation, or ChIP; miRNA high-throughput profiling and the in situ detection of miRNA subtle expression in the brain; analysis of genes with alternative 3’UTRs; and the cite-specific delivery of chromatin-modifying drugs. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.


Concise and easy-to-use, Epigenetic Methods in Neuroscience Research provides multidisciplinary epigenetic approach to study genome, and neural, plasticity that will help the reader to successfully address the challenges associated with neurodevelopmental, psychiatric, and neurodegenerative disorders.


ChIP DNA chromatin immunoprecipitation cytosines global analyzes of DNA methylation liquid chromatography/mass spectrometry non-coding RNA analyses non-coding genome single-gene DNA methylation spatial DNA methylation patterns

Editors and affiliations

  • Nina Karpova
    • 1
  1. 1.Neuroscience CenterUniversity of HelsinkiHelsinkiFinland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2753-1
  • Online ISBN 978-1-4939-2754-8
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site
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