Yeast Surface Display

Methods, Protocols, and Applications

  • Bin Liu

Part of the Methods in Molecular Biology book series (MIMB, volume 1319)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Yeast Surface Antibody Display Library and Antibody Engineering

    1. Front Matter
      Pages 1-1
    2. Alessandro Angelini, Tiffany F. Chen, Seymour de Picciotto, Nicole J. Yang, Alice Tzeng, Michael S. Santos et al.
      Pages 3-36
    3. Benjamin J. Tillotson, Jason M. Lajoie, Eric V. Shusta
      Pages 65-78
  3. Yeast Surface Display as a Tool for Protein Engineering

    1. Front Matter
      Pages 79-79
    2. Li Yi, Joseph M. Taft, Qing Li, Mark C. Gebhard, George Georgiou, Brent L. Iverson
      Pages 81-93
    3. Sheena N. Smith, Daniel T. Harris, David M. Kranz
      Pages 95-141
    4. Jasdeep K. Mann, Sheldon Park
      Pages 143-154
    5. Gerald M. Cherf, Jennifer R. Cochran
      Pages 155-175
  4. Yeast Surface cDNA Display Library Construction and Applications

  5. Yeast Surface Display in Bioassay and Industrial Applications

    1. Front Matter
      Pages 215-215
    2. Natsuko Miura, Kouichi Kuroda, Mitsuyoshi Ueda
      Pages 217-232
    3. Hongwei Wang, Qiaolin Lang, Bo Liang, Aihua Liu
      Pages 233-243
    4. Keya Zhang, Karan Bhuripanyo, Yiyang Wang, Jun Yin
      Pages 245-260
    5. Alon Szczupak, Lital Alfonta
      Pages 261-268
  6. Back Matter
    Pages 269-271

About this book


In addition to research and discovery, yeast surface display technology has found applications in industrial processes such as biofuel production and environmental pollutant absorption and degradation. Yeast Surface Display: Methods, Protocols, and Applications guides readers through yeast surface antibody display library and antibody engineering,  yeast surface display as a tool for protein engineering, yeast surface cDNA display library construction and applications, and yeast surface display in bioassay and industrial applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Concise and easy-to-use, Yeast Surface Display: Methods, Protocols, and Applications aims to help accelerate the work of protein chemists, antibody engineers, molecular and cell biologists, and industrial bioengineers. ​


T cell receptors antibody library screening cDNA fragment cellular proteins binding epitope mapping novel protein-ligand interactions protein and antibody engineering protein engineering surface of yeast

Editors and affiliations

  • Bin Liu
    • 1
  1. 1.UCSFSan FranciscoUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2747-0
  • Online ISBN 978-1-4939-2748-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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