Detection of Blotted Proteins

Methods and Protocols

  • Biji T. Kurien
  • R. Hal Scofield

Part of the Methods in Molecular Biology book series (MIMB, volume 1314)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Vishal Kothari, Suresh T. Mathews
    Pages 27-32
  3. Anil Dsouza, R. Hal Scofield
    Pages 33-40
  4. F. Javier Alba, Salvador Bartolomé, Antonio Bermúdez, Joan-Ramon Daban
    Pages 41-50
  5. Neil W. Charter, Joe Horecka, Chin-Yee Loh, Albert Doan, Tom Wehrman, Keith R. Olson
    Pages 51-61
  6. Claudia Franke, Daniel Gräfe, Holger Bartsch, Michael P. Bachmann
    Pages 63-71
  7. Holger Bartsch, Claudia Franke, Michael P. Bachmann
    Pages 73-78
  8. Surbhi Desai, Boguslawa R. Dworecki, Marie C. Nlend
    Pages 101-114
  9. Caitriona Scaife, Ciara A. McManus, Pamela M. Donoghue, Michael J. Dunn
    Pages 139-149
  10. Biji T. Kurien, Michael P. Bachmann
    Pages 165-171
  11. Jun Park, Masaharu Mabuchi, Ajay Sharma
    Pages 191-194
  12. Niels Grützner, Romy M. Heilmann, Aaron G. Smith, Carol B. Johnson, Stanislav Vitha, Jörg M. Steiner et al.
    Pages 207-217
  13. Jose L. Luque-Garcia, Thomas A. Neubert
    Pages 243-253
  14. Biji T. Kurien, Michael P. Bachmann
    Pages 255-261
  15. Biji T. Kurien, R. Hal Scofield
    Pages 273-277
  16. Jayanta K. Pal, Shilpa J. Rao, Dhanashri J. Godbole
    Pages 279-285
  17. Stan (Stanislaw) Krajewski, Michelle M. Tsukamoto, Xianshu Huang, Sebastian B. Krajewski
    Pages 287-301
  18. Yu-ki Matsuno, Akihiko Kameyama
    Pages 303-311
  19. Akihiko Kameyama, Weijie Dong, Yu-ki Matsuno
    Pages 325-331
  20. Suresh T. Mathews, Emily Graff, Robert L. Judd, Vishal Kothari
    Pages 333-348
  21. Parmita Kar, Saurabh Kumar Agnihotri, Archana Sharma, Rekha Sachan, Madan Lal Bhatt, Monika Sachdev
    Pages 349-355
  22. Biji T. Kurien, R. Hal Scofield
    Pages 357-370
  23. Jasmin R. Kurien, Bianca A. Kurien
    Pages 371-374
  24. Biji T. Kurien
    Pages 375-382
  25. Back Matter
    Pages 383-386

About this book


This detailed volume provides methods and techniques for detection after blotting.  Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.


detecting blotted molecular species detecting the blotted proteins protein blotting protein transfer western blotting techniques

Editors and affiliations

  • Biji T. Kurien
    • 1
  • R. Hal Scofield
    • 2
  1. 1.Arthritis and Clinical Immunology ProgramOklahoma Medical Research FoundationOklahoma CityUSA
  2. 2.Department of MedicineOklahoma Medical Research FoundationOklahoma CityUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2717-3
  • Online ISBN 978-1-4939-2718-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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