Western Blotting

Methods and Protocols

  • Biji T. Kurien
  • R. Hal Scofield

Part of the Methods in Molecular Biology book series (MIMB, volume 1312)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Edwin Southern
    Pages 1-3
  3. Harry Towbin
    Pages 5-7
  4. W. Neal Burnette
    Pages 9-12
  5. Jaime Renart
    Pages 13-16
  6. Biji T. Kurien, R. Hal Scofield
    Pages 17-30
  7. Biji T. Kurien, Harry Towbin
    Pages 31-40
  8. Mandy Peach, Noelle Marsh, Ewa I. Miskiewicz, Daniel J. MacPhee
    Pages 49-60
  9. Graeme Lowe
    Pages 87-92
  10. Rafael F. Pont-Lezica
    Pages 93-104
  11. Sumi Surendran, Annamma Mathai, Vishnampet Venkataraman Radhakrishnan
    Pages 105-108
  12. Biji T. Kurien, R. Hal Scofield
    Pages 109-117
  13. Dalit Talmi-Frank, Charles L. Jaffe, Gad Baneth
    Pages 141-150
  14. Engelbert Buxbaum
    Pages 151-163
  15. Ulrich Canzler, Holger Bartsch, Kai Großmann, Werner Lehmann, Karsten Conrad, Biji T. Kurien et al.
    Pages 165-173
  16. Edita Aksamitiene, Jan B. Hoek, Anatoly Kiyatkin
    Pages 197-226
  17. Joseph Kost, Aharon Azagury
    Pages 237-246
  18. Biji T. Kurien, R. Hal Scofield
    Pages 247-255
  19. Oscar Noya, Sandra Losada, Marilyan Toledo, Adriana Gauna, María Angelita Lorenzo, Henry Bermúdez et al.
    Pages 301-319
  20. Edward P. Trieu, Ira N. Targoff
    Pages 327-342
  21. Ioannis N. Manoussopoulos, Mina Tsagris
    Pages 343-353
  22. Olga Yeliosof, R. Hal Scofield
    Pages 375-378
  23. Joshua A. Jadwin, Bruce J. Mayer, Kazuya Machida
    Pages 379-398
  24. Jinny Paul
    Pages 411-414
  25. Biji T. Kurien, Debashish Danda, Michael P. Bachmann, R. Hal Scofield
    Pages 449-454
  26. Syed M. S. Quadri
    Pages 455-464
  27. Valerie M. Harris
    Pages 465-468
  28. Pothur R. Srinivas
    Pages 469-472
  29. Hisao Haniu, Daisuke Watanabe, Yusuke Kawashima, Hiroyuki Matsumoto
    Pages 473-479

About this book


This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.



Antigenic Peptides Centrifuge Blotting Diffusion Blotting Electroblotting Immunoprecipitation Protein Slice Blotting Western Immublotting

Editors and affiliations

  • Biji T. Kurien
    • 1
  • R. Hal Scofield
    • 2
  1. 1.Department of Medicine, University of OklahomaArthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of VeteransOklahoma CityUSA
  2. 2.Department of MedicineArthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical CenterOklahoma CityUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2693-0
  • Online ISBN 978-1-4939-2694-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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