Pulse Field Gel Electrophoresis

Methods and Protocols

  • Kieran Jordan
  • Marion Dalmasso

Part of the Methods in Molecular Biology book series (MIMB, volume 1301)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Introduction to PFGE

  3. PFGE and Pathogenic Bacteria

    1. Front Matter
      Pages 41-41
    2. Laura Luque-Sastre, Séamus Fanning, Edward M. Fox
      Pages 43-53
    3. George R. Golding, Jennifer Campbell, Dave Spreitzer, Linda Chui
      Pages 85-93
    4. Wondwossen A. Gebreyes, Pamela R. F. Adkins
      Pages 95-101
    5. Carolina Lúquez, Lavin A. Joseph, Susan E. Maslanka
      Pages 103-113
    6. Tamara Revazishvili, Judith A. Johnson
      Pages 115-128
    7. Luca Agostino Vitali, Giovanni Gherardi, Dezemona Petrelli
      Pages 129-138
    8. Patricia Jaros, Muriel Dufour, Brent Gilpin, Molly M. Freeman, Efrain M. Ribot
      Pages 171-189
    9. Antonio Camarda, Elena Circella, Antonia Pupillo, Marilisa Legretto, Michele Marino, Nicola Pugliese
      Pages 191-210
  4. PFGE and Non-pathogenic Bacteria

    1. Front Matter
      Pages 211-211
    2. Pascal Le Bourgeois, Delphine Passerini, Michèle Coddeville, Maéva Guellerin, Marie-Line Daveran-Mingot, Paul Ritzenthaler
      Pages 213-224
    3. Esther Jiménez, Marta Gómez, Laura Moles
      Pages 253-263
    4. Victoria Chuat, Rosangela de Freitas, Marion Dalmasso
      Pages 265-275
  5. Back Matter
    Pages 277-278

About this book


This  volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.


Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.


DNA fragments PFGE PulseNet disease monitoring epidemiological investigations genome sequencing genomics metagenomics non-pathogenic bacteria pathogenic bacteria

Editors and affiliations

  • Kieran Jordan
    • 1
  • Marion Dalmasso
    • 2
  1. 1.Teagasc Food Research Center, Moorepark, FermoyCo. CorkIreland
  2. 2.Department of Microbiology & Alimentary Pharmabiotic CentreUniversity College CorkCo. CorkIreland

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2598-8
  • Online ISBN 978-1-4939-2599-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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