RNA Nanotechnology and Therapeutics

Methods and Protocols

  • Peixuan Guo
  • Farzin Haque

Part of the Methods in Molecular Biology book series (MIMB, volume 1297)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Christophe Lavelle, Florent Busi, Véronique Arluison
    Pages 21-42
  3. Kyung Duk Koh, Hsiang-Chih Chiu, Elisa Riedo, Francesca Storici
    Pages 43-57
  4. Kirill A. Afonin, Danielle Schultz, Luc Jaeger, Elisabeth Gwinn, Bruce A. Shapiro
    Pages 59-66
  5. Daniel L. Jasinski, Chad T. Schwartz, Farzin Haque, Peixuan Guo
    Pages 67-82
  6. Zhen Huang, Chi-Yen Lin, William Jaremko, Li Niu
    Pages 83-93
  7. Chen Qiu, Wang-Yi Liu, Yong-Zhen Xu
    Pages 113-120
  8. Piotr Rychahou, Yi Shu, Farzin Haque, Jiyao Hu, Peixuan Guo, B. Mark Evers
    Pages 121-135
  9. Tae Jin Lee, Farzin Haque, Mario Vieweger, Ji Young Yoo, Balveen Kaur, Peixuan Guo et al.
    Pages 137-152
  10. Laetitia Evadé, Eric Dausse, Said Taouji, Emilie Daguerre, Eric Chevet, Jean-Jacques Toulmé
    Pages 153-167
  11. Hirohisa Ohno, Eriko Osada, Hirohide Saito
    Pages 197-211
  12. Baochang Fan, Peng Ni, C. Cheng Kao
    Pages 213-224
  13. Back Matter
    Pages 237-239

About this book


This volume contains a compilation of techniques and laboratory protocols directly related to RNA nanotechnology and its applications in nanobiotechnology and nanomedicine.  The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nanoarchitectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nanoconstructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubles troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.


Nanobiotechnology Nanomedicine RNA assembly RNA folding RNA nanoarchitectures RNA-protein nanostructures ultracentrifugation

Editors and affiliations

  • Peixuan Guo
    • 1
  • Farzin Haque
    • 2
  1. 1.Nanobiotechnology Center, Markey Cancer Center, Department of Pharmaceutical SciencesUniversity of KentuckyLexingtonUSA
  2. 2.Nanobiotechnology Center, Markey Cancer Center, Department of Pharmaceutical SciencesUniversity of KentuckyLexingtonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2561-2
  • Online ISBN 978-1-4939-2562-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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