cAMP Signaling

Methods and Protocols

  • Manuela Zaccolo

Part of the Methods in Molecular Biology book series (MIMB, volume 1294)

Table of contents

  1. Front Matter
    Pages i-x
  2. Silas J. Leavesley, Arie Nakhmani, Yi Gao, Thomas C. Rich
    Pages 59-70
  3. Thomas C. Rich, Wenkuan Xin, Silas J. Leavesley, Mark S. Taylor
    Pages 71-84
  4. Oliver Lomas, Marcella Brescia, Ricardo Carnicer, Stefania Monterisi, Nicoletta C. Surdo, Manuela Zaccolo
    Pages 103-115
  5. Daniela Hübscher, Viacheslav O. Nikolaev
    Pages 117-129
  6. Eileen J. Kennedy, John D. Scott
    Pages 137-150
  7. Carolin Schächterle, Frank Christian, João Miguel Parente Fernandes, Enno Klussmann
    Pages 151-166
  8. Ana Santos de Medeiros, Charles S. Hoffman
    Pages 181-190
  9. Eleonora Corradini, Albert J. R. Heck, Arjen Scholten
    Pages 191-201
  10. Susana R. Neves-Zaph, Roy S. Song
    Pages 203-217
  11. Back Matter
    Pages 219-222

About this book


This detailed volume encompasses new technological developments that specifically address questions related to adenosine 3′,5′-monophosphate (cAMP) compartmentalization, that probe relevant protein-protein interactions, that increase the spatial and temporal resolution of cAMP signal detection, and that can facilitate integration of the mounting complexity of the information that is becoming available on this signaling system. cAMP, the prototypical intracellular second messenger, regulates a large variety of cellular functions and biological processes, including gene transcription, cell metabolism, proliferation, development, as well as more specialized functions depending on the cell type, so the realization of its extremely complex spatial organization and local regulation is providing novel mechanistic insight into cell physiology and is producing a novel framework for the identification of disease mechanisms. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and authoritative, cAMP Signaling: Methods and Protocols serves as a vital resource for researchers working in this expanding, complex field.


Adenosine 3′,5′-monophosphate Cellular function Disease mechanisms Protein kinase A (PKA) Signaling pathways cAMP signals

Editors and affiliations

  • Manuela Zaccolo
    • 1
  1. 1.Department of Physiology, Anatomy, and GeneticsUniversity of OxfordOxfordUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2536-0
  • Online ISBN 978-1-4939-2537-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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