T follicular Helper Cells

Methods and Protocols

  • Marion Espéli
  • Michelle Linterman

Part of the Methods in Molecular Biology book series (MIMB, volume 1291)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Mouse

    1. Front Matter
      Pages 1-1
    2. Alexandre P. Meli, Irah L. King
      Pages 3-11
    3. Yanna Ding, John D. Mountz, Hui-Chen Hsu
      Pages 13-25
    4. Dirk Baumjohann, K. Mark Ansel
      Pages 27-38
    5. Youn Soo Choi, Shane Crotty
      Pages 49-61
    6. R. Brink, D. Paus, K. Bourne, J. R. Hermes, S. Gardam, T. G. Phan et al.
      Pages 103-123
    7. Giulia Pasqual, Alessandro Angelini, Gabriel D. Victora
      Pages 125-134
  3. Human

    1. Front Matter
      Pages 161-161
    2. Chang H. Kim, Seika Hashimoto-Hill, Seung G. Kang
      Pages 175-186
    3. Salah-Eddine Bentebibel, Clement Jacquemin, Nathalie Schmitt, Hideki Ueno
      Pages 187-197
  4. Back Matter
    Pages 227-228

About this book


This volume brings together the skills and protocols of numerous laboratories that are at the heart of investigation into the biology of Tfh cells in both mice and humans.  As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.


Concise and easy-to-use, T follicular Helper Cells: Methods and Protocols provides scientist with techniques and protocols that have facilitated breakthroughs in Tfh biology and to present them in a way that will enable both new and experienced researchers to continue to move this exciting field forward.


CD4+ T cells T follicular helper cells Tfh biology cell culture germinal centre immunologists phenotyped transcriptomics

Editors and affiliations

  • Marion Espéli
    • 1
  • Michelle Linterman
    • 2
  1. 1.INSERM UMR_S996, LabEx LERMITUniversité Paris-SudClamartFrance
  2. 2.Lymphocyte Signalling and Development ISP Babraham InstituteBabraham Research CampusCambridgeUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2497-4
  • Online ISBN 978-1-4939-2498-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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