Salamanders in Regeneration Research

Methods and Protocols

  • Anoop Kumar
  • András Simon

Part of the Methods in Molecular Biology book series (MIMB, volume 1290)

Table of contents

  1. Front Matter
    Pages i-x
  2. Salamanders

    1. Front Matter
      Pages 1-1
    2. Johanna E. Farkas, James R. Monaghan
      Pages 27-46
    3. Alberto Joven, Matthew Kirkham, András Simon
      Pages 47-70
    4. Claudia Marcela Arenas, Andrea Gómez-Molina, Jean Paul Delgado
      Pages 71-78
  3. Experimental Manipulation in Salamanders

    1. Front Matter
      Pages 79-79
    2. Tetsuya Endo, David M. Gardiner, Aki Makanae, Akira Satoh
      Pages 101-113
    3. Aida Rodrigo Albors, Elly M. Tanaka
      Pages 115-125
    4. Tzu-Hsing Kuo, Jessica L. Whited
      Pages 127-140
    5. Peggy S. Coots, Ashley W. Seifert
      Pages 141-145
    6. Anoop Kumar, Jean Paul Delgado
      Pages 147-157
  4. Salamander Cells in Culture

    1. Front Matter
      Pages 169-169
    2. Jean-François Denis, Fadi Sader, Patrizia Ferretti, Stéphane Roy
      Pages 187-196
    3. Liyakath Ali Shahul Hameed, András Simon
      Pages 197-204
    4. Tanja Piatkowski, Thomas Braun
      Pages 241-251
  5. Transgenesis in Salamanders

    1. Front Matter
      Pages 267-267
    2. Shahryar Khattak, Elly M. Tanaka
      Pages 269-277
  6. Gene Expression

    1. Front Matter
      Pages 307-307
    2. S. Randal Voss, Antony Athippozhy, M. Ryan Woodcock
      Pages 309-319
    3. Nour W. Al Haj Baddar, M. Ryan Woodcock, Shivam Khatri, D. Kevin Kump, S. Randal Voss
      Pages 321-336
  7. Back Matter
    Pages 353-357

About this book


This detailed volume focuses on best practices and conditions for maintaining the most commonly used salamander species in the laboratory. Salamanders in Regeneration Research: Methods and Protocols guides readers through experimental manipulations in vivo and in vitro, respectively. With methods on targeting a wide variety of structures, ranging from the limb to the heart and to the brain, and methods for studying genetically modified organisms and tools for mining in the genomic databases. Written in the highly successful Methods in Molecular Biology series format, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and up-to-date, Salamanders in Regeneration Research: Methods and Protocols provides a comprehensive collection of methods chapters.


brain evolutionary genetically modified organisms genomic databases heart in vitro in vivo phylogenetic salamander regeneration salamander species

Editors and affiliations

  • Anoop Kumar
    • 1
  • András Simon
    • 2
  1. 1.Institute of Structural and Molecular Biology, Division of BiosciencesUniversity College LondonLondonUnited Kingdom
  2. 2.Department of Cell and Molecular BiologyKarolinska InstituteStockholmSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2494-3
  • Online ISBN 978-1-4939-2495-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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