Methods and Protocols

  • Helena Jane Maier
  • Erica Bickerton
  • Paul Britton

Part of the Methods in Molecular Biology book series (MIMB, volume 1282)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Anthony R. Fehr, Stanley Perlman
    Pages 1-23
  3. Detection, Diagnosis and Evolution of Coronaviruses

  4. Propogation, Titration, and Purification of Coronaviruses

  5. Manipulating the Genomes of Coronaviruses

    1. Front Matter
      Pages 113-113
    2. Fernando Almazán, Silvia Márquez-Jurado, Aitor Nogales, Luis Enjuanes
      Pages 135-152
  6. Coronavirus Attachment and Entry

    1. Front Matter
      Pages 153-153
    2. V. Stalin Raj, Mart M. Lamers, Saskia L. Smits, Jeroen A. A. Demmers, Huihui Mou, Berend-Jan Bosch et al.
      Pages 165-182
    3. Deirdre A. Costello, Susan Daniel
      Pages 183-194
  7. Studying Virus-Host Interactions

    1. Front Matter
      Pages 195-195
    2. Chee-Hing Yang, Hui-Chun Li, Cheng-Huei Hung, Shih-Yen Lo
      Pages 197-212
    3. Pierre-Olivier Vidalain, Yves Jacob, Marne C. Hagemeijer, Louis M. Jones, Grégory Neveu, Jean-Pierre Roussarie et al.
      Pages 213-229
    4. Parvaneh Mehrbod, Mohammad Syamsul Reza Harun, Ahmad Naqib Shuid, Abdul Rahman Omar
      Pages 241-250
    5. Joeri Kint, Maria Forlenza
      Pages 251-259
    6. Marne C. Hagemeijer, Cornelis A. M. de Haan
      Pages 261-269
  8. Back Matter
    Pages 283-285

About this book


This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.


coronovirology titration techniques viral protein virus genomes virus pathogenicity virus replication virus-cell fusion virus-host interactions

Editors and affiliations

  • Helena Jane Maier
    • 1
  • Erica Bickerton
    • 2
  • Paul Britton
    • 3
  1. 1.The Pirbright InstituteComptonUnited Kingdom
  2. 2.The Pirbright InstituteComptonUnited Kingdom
  3. 3.The Pirbright InstituteComptonUnited Kingdom

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2437-0
  • Online ISBN 978-1-4939-2438-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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