Protein-Protein Interactions

Methods and Applications

  • Cheryl L. Meyerkord
  • Haian Fu

Part of the Methods in Molecular Biology book series (MIMB, volume 1278)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Overviews

    1. Front Matter
      Pages 1-1
    2. Robert C. Liddington
      Pages 3-22
    3. Ziad M. Eletr, Keith D. Wilkinson
      Pages 23-37
    4. Damian Szklarczyk, Lars Juhl Jensen
      Pages 39-56
    5. Tobias Ehrenberger, Lewis C. Cantley, Michael B. Yaffe
      Pages 57-75
  3. Label-Free Biosensors and Techniques

    1. Front Matter
      Pages 107-107
    2. Gabriella T. Heller, Alison R. Mercer-Smith, Malkiat S. Johal
      Pages 153-164
    3. Adrian Velazquez-Campoy, Stephanie A. Leavitt, Ernesto Freire
      Pages 183-204
    4. Ian A. Taylor, Katrin Rittinger, John F. Eccleston
      Pages 205-222
    5. Peter M. Thompson, Moriah R. Beck, Sharon L. Campbell
      Pages 267-279
    6. Vishwajeeth R. Pagala, Anthony A. High, Xusheng Wang, Haiyan Tan, Kiran Kodali, Ashutosh Mishra et al.
      Pages 281-305
  4. Tag/Affinity-Based Methods

  5. Cell-Based Bimolecular Interaction Reporter Assays

    1. Front Matter
      Pages 407-407
    2. Matthew A. Bennett, Jack F. Shern, Richard A. Kahn
      Pages 433-446
    3. Nicole E. Brown, Joe B. Blumer, John R. Hepler
      Pages 457-465
    4. Ingrid Remy, Stephen W. Michnick
      Pages 467-481
    5. Alexander S. Shavkunov, Syed R. Ali, Neli I. Panova-Elektronova, Fernanda Laezza
      Pages 497-514
  6. High Throughput Screening Assays for Protein-Protein Interactions: Case Studies

    1. Front Matter
      Pages 527-527
    2. Terry W. Moore, Jillian R. Gunther, John A. Katzenellenbogen
      Pages 545-553
    3. Liu Liu, Denzil Bernard, Shaomeng Wang
      Pages 567-585
    4. Samuel J. Pfaff, Michael S. Chimenti, Mark J. S. Kelly, Michelle R. Arkin
      Pages 587-613
  7. Back Matter
    Pages 615-620

About this book


In recent years, our understanding of the protein-protein interaction landscape has expanded significantly. Growing interest in analyzing and targeting protein-protein interactions has resulted in an increased need for detailed methodologies to interrogate and monitor protein-protein interactions. To meet this need, Protein-Protein Interactions: Methods and Applications has been updated and expanded. The second edition includes core technological platforms used to study protein-protein interactions, and cutting-edge technologies that reflect recent scientific advances and the emerging focus on therapeutic discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of necessary materials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. These well-detailed protocols describe methods for identifying protein-protein interaction partners, analyzing protein-protein interactions quantitatively and qualitatively, monitoring protein-protein interactions in live cells, and predicting and determining interaction interfaces.

Authoritative and cutting-edge, Protein-Protein Interactions: Methods and Applications, Second Edition is a valuable resource that will enable readers to elucidate the mechanisms of protein-protein interactions, determine the role of these interactions in diverse biological processes, and target protein-protein interactions for therapeutic discovery. 


cell-based bimolecular interaction drug discovery fluorophore labeling of test proteins label-free biosensors and techniques protein-protein interactions quantitative and computational analysis small molecule modulator identification tag- and antibody-based methods traditional radioisotope

Editors and affiliations

  • Cheryl L. Meyerkord
    • 1
  • Haian Fu
    • 2
  1. 1.Department of Pharmacology and Emory Chemical Biology Discovery CenterEmory University School of MedicineAtlantaUSA
  2. 2.Department of Pharmacology, Department of Hematology & Medical Oncology, and Emory Chemical Biology Discovery CenterEmory University School of MedicineAtlantaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2424-0
  • Online ISBN 978-1-4939-2425-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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