Gene Essentiality

Methods and Protocols

  • Long Jason Lu

Part of the Methods in Molecular Biology book series (MIMB, volume 1279)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Hirotada Mori, Tomoya Baba, Katsushi Yokoyama, Rikiya Takeuchi, Wataru Nomura, Kazuichi Makishi et al.
    Pages 45-65
  3. Thanatchaporn Bartpho, Gerald L. Murray
    Pages 67-77
  4. Jarukit E. Long, Michael DeJesus, Doyle Ward, Richard E. Baker, Thomas Ioerger, Christopher M. Sassetti
    Pages 79-95
  5. Mark Reuter, Duncan J. H. Gaskin, Aline Metris
    Pages 167-181
  6. Feng-Biao Guo, Yuan-Nong Ye, Lu-Wen Ning, Wen Wei
    Pages 205-217
  7. Feng Gao, Hao Luo, Chun-Ting Zhang, Ren Zhang
    Pages 219-233
  8. Yulan Lu, Yao Lu, Jingyuan Deng, Hui Lu, Long Jason Lu
    Pages 235-245
  9. Back Matter
    Pages 247-248

About this book


This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.


Candida albicans Computational predictions Microbial essential genes Prokaryotes Single-gene knockouts Transposon mutagenesis

Editors and affiliations

  • Long Jason Lu
    • 1
  1. 1.Cincinnati Children’s Hospital Medical CenterCincinnatiUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2397-7
  • Online ISBN 978-1-4939-2398-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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