G Protein-Coupled Receptor Screening Assays

Methods and Protocols

  • Duarte Miguel F. Prazeres
  • Sofia Aires M. Martins

Part of the Methods in Molecular Biology book series (MIMB, volume 1272)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Duarte Miguel F. Prazeres, Sofia Aires M. Martins
      Pages 3-19
  3. GPCR Screening Assays

    1. Front Matter
      Pages 21-21
    2. Nadia Oueslati, Candide Hounsou, Abderazak Belhocine, Thieric Rodriguez, Elodie Dupuis, Jurriaan M. Zwier et al.
      Pages 23-36
    3. Olga Mazina, Anni Allikalt, Annika Heinloo, Reet Reinart-Okugbeni, Sergei Kopanchuk, Ago Rinken
      Pages 65-77
    4. Grzegorz Woszczek, Elisabeth Fuerst
      Pages 79-89
    5. Macarena Pampillo, Andy V. Babwah
      Pages 119-132
    6. Francisco Ciruela, Víctor Fernández-Dueñas
      Pages 133-141
    7. João R. C. Trabuco, Sofia Aires M. Martins, Duarte Miguel F. Prazeres
      Pages 143-172
    8. Laura Martínez-Muñoz, Rubén Barroso, Anabel Guedán Paredes, Mario Mellado, José Miguel Rodríguez-Frade
      Pages 173-188
    9. Jong Hyun Lim, Juhun Park, Seunghun Hong, Tai Hyun Park
      Pages 189-198
    10. Manuel Grundmann, Evi Kostenis
      Pages 199-213
    11. Ning Ke, Khanh Nguyen, Jeffery Irelan, Yama A. Abassi
      Pages 215-226
    12. Ann M. Ferrie, Vasiliy Goral, Chaoming Wang, Ye Fang
      Pages 227-246
    13. Hideo Takakura, Mitsuru Hattori, Miho Tanaka, Takeaki Ozawa
      Pages 257-270
    14. Hugo Gutiérrez-de-Terán, Henrik Keränen, Jhonny Azuaje, David Rodríguez, Johan Åqvist, Eddy Sotelo
      Pages 271-291
  4. Back Matter
    Pages 293-296

About this book


 This volume explores the considerable efforts that have been directed towards the development of G Protein-Coupled Receptors (GPCR) screening assays in order to disclose GPCR acting compounds, elucidate signaling mechanisms or evaluate compound’s efficacy. New discoveries in the field, along with the widely recognized need for better and safer pharmaceutical drugs constitute the main motivation for this book. Readers, both beginners and experienced researchers, will receive an updated overview of not only the established, but also the innovative technologies that promise to advance GPCR drug research. This book is organized into two major parts: the introductory part discusses the necessary foundations for the understanding of GPCR action and the rationale behind the design of the available screening assays; and part two provides detailed protocols for different screening approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Practical and innovative, G Protein-Coupled Receptor Screening Assays: Methods and Protocols reaches out to everyone involved in the discovery of GPCR-active drugs, and provides a transversal overview of the different levels of GPCR signaling addressable in the different screening strategies and presents practical examples of how current assay technologies are contributing to new paradigms in GPCR drug research.


G-Protein Coupled Receptors GPCR signaling GPCR-active drugs drug research high content screening high throughput ligand membrane protein screening assays

Editors and affiliations

  • Duarte Miguel F. Prazeres
    • 1
    • 2
  • Sofia Aires M. Martins
    • 1
  1. 1.IBB – Institute for Biotechnology and BioengineeringLisbonPortugal
  2. 2.Department of BioengineeringInstituto Superior Técnico Universidade de LisboaLisbonPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2335-9
  • Online ISBN 978-1-4939-2336-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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