Nuclear Bodies and Noncoding RNAs

Methods and Protocols

  • Shinichi Nakagawa
  • Tetsuro Hirose

Part of the Methods in Molecular Biology book series (MIMB, volume 1262)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Imaging of Noncoding RNAs and Nuclear Bodies

    1. Front Matter
      Pages 1-1
    2. Margaret Dunagin, Moran N. Cabili, John Rinn, Arjun Raj
      Pages 3-19
    3. Yasushi Okada, Shinichi Nakagawa
      Pages 21-35
    4. Saskia Hutten, Samuel Swift, Angus I. Lamond
      Pages 55-67
    5. Tokuko Haraguchi, Hiroko Osakada, Takako Koujin
      Pages 89-103
    6. Sylvie Souquere, Gérard Pierron
      Pages 105-118
    7. Jamie L. Gilmore, Aiko Yoshida, Hirohide Takahashi, Katashi Deguchi, Toshiro Kobori, Emilie Louvet et al.
      Pages 119-153
  3. Biochemical Analysis of Noncoding RNAs and Nuclear Bodies

  4. Cell and Molecular Biological Approach Toward the Analysis of Noncoding RNAs and Nuclear Bodies

    1. Front Matter
      Pages 275-275
    2. Shingo Kose, Tomoko Funakoshi, Naoko Imamoto
      Pages 291-303
    3. Hidenori Tani, Naoto Imamachi, Rena Mizutani, Katsutoshi Imamura, Yeondae Kwon, Satoru Miyazaki et al.
      Pages 305-320
    4. Xinying Zong, Lulu Huang, Vidisha Tripathi, Raechel Peralta, Susan M. Freier, Shuling Guo et al.
      Pages 321-331
    5. Yuko Hasegawa, Shinichi Nakagawa
      Pages 333-348
  5. Back Matter
    Pages 349-351

About this book


This volume focuses on cytological, biochemical, and molecular biological methods to identify and examine the function of each nuclear body, with an emphasis on the analysis of long non-coding RNAs. Chapters focus on exploring recent studies that reveal how certain long non protein-coding RNAs accumulate in specific nuclear bodies and regulate the function of the bodies by serving as architectural components or controlling the dynamics of associating protein components. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Nuclear Bodies and Noncoding RNAs: Methods and Protocols serves as a guideline for further study into the sophisticated regulation of gene expression.


biochemical cytological eukaryotes gene expression long non-coding RNAs molecular biological methods non protein-coding RNAs nuclear body ribonucleoprotein complexes

Editors and affiliations

  • Shinichi Nakagawa
    • 1
  • Tetsuro Hirose
    • 2
  1. 1.RNA Biology LaboratoryRIKENSaitamaJapan
  2. 2.Institute for Genetic MedicineHokkaido UniversitySapporoJapan

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2252-9
  • Online ISBN 978-1-4939-2253-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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