Cryopreservation and Freeze-Drying Protocols

  • Willem F. Wolkers
  • Harriëtte Oldenhof

Part of the Methods in Molecular Biology book series (MIMB, volume 1257)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Fundamental Aspects and Freezing Technology

    1. Front Matter
      Pages 1-1
    2. David E. Pegg
      Pages 3-19
    3. Gregory M. Fahy, Brian Wowk
      Pages 21-82
    4. James D. Benson
      Pages 83-120
    5. Gerald D. J. Adams, Isobel Cook, Kevin R. Ward
      Pages 121-143
  3. Methods to Study Freezing and Drying Processes

    1. Front Matter
      Pages 145-145
    2. Frank Stracke, Asger Kreiner-Møller, Heiko Zimmermann
      Pages 229-241
  4. Cryopreservation Protocols

    1. Front Matter
      Pages 275-275
    2. Harald Sieme, Harriëtte Oldenhof
      Pages 277-287
    3. Victoria Keros, Barry J. Fuller
      Pages 289-304
    4. Julia C. Neubauer, Axel F. Beier, Niels Geijsen, Heiko Zimmermann
      Pages 321-328
    5. Serean L. Adams, John F. Smith, Jolene Taylor, Lindsay T. McGowan, H. Robin Tervit
      Pages 329-336
    6. Phillip H. Purdy, James K. Graham
      Pages 337-342
    7. Harald Sieme, Harriëtte Oldenhof
      Pages 343-352
    8. Johan W. Lagerberg
      Pages 353-367
    9. Heidi Elmoazzen, Jelena L. Holovati
      Pages 369-379
    10. Kelvin G. M. Brockbank, Zhenzhen Chen, Elizabeth D. Greene, Lia H. Campbell
      Pages 399-421
    11. Heinz Martin Schumacher, Martina Westphal, Elke Heine-Dobbernack
      Pages 423-429
  5. Freeze-Drying Protocols

    1. Front Matter
      Pages 457-457
    2. Baolin Liu, Xinli Zhou
      Pages 459-476
    3. Fernanda Fonseca, Stéphanie Cenard, Stéphanie Passot
      Pages 477-488
    4. Levent Keskintepe, Ali Eroglu
      Pages 489-497
    5. Willem F. Wolkers, Andres Hilfiker
      Pages 499-506
  6. Back Matter
    Pages 507-509

About this book


This volume provides a variety of standard protocols used to cryopreserve or freeze-dry different types of specimens. In addition, it provides chapters focused on the fundamental principles of cryopreservation, vitrification, and freeze-drying. Several state of the art microscopic, spectroscopic as well as calorimetric methods are highlighted that can be used to study cellular and macromolecular changes in response to freezing or drying. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cryopreservation and Freeze-Drying Protocols, Third Edition serves as a practical guideline for studies on freezing and drying processes as well as preservation strategies for biological specimens.


Cryopreservation calorimetric methods cellular changes freeze-drying macromolecular changes microscopic methods oocytes spectroscopic methods sperm vitrification

Editors and affiliations

  • Willem F. Wolkers
    • 1
  • Harriëtte Oldenhof
    • 2
  1. 1.Institute of Multiphase ProcessesLeibniz Universität HannoverHannoverGermany
  2. 2.Unit for Reproductive Medicine, Clinic for HorsesUniversity of Veterinary Medicine HannoverHannoverGermany

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2192-8
  • Online ISBN 978-1-4939-2193-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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