Protocols in In Vitro Hepatocyte Research

  • Mathieu Vinken
  • Vera Rogiers

Part of the Methods in Molecular Biology book series (MIMB, volume 1250)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. In Vitro Models of the Hepatocyte Life Cycle

    1. Front Matter
      Pages 1-1
    2. Lyndsey Houseman, Mina Edwards, Ian R. Phillips, Elizabeth A. Shephard
      Pages 3-12
    3. Nicola J. Hewitt, Albert P. Li
      Pages 13-26
    4. Eva Ramboer, Tamara Vanhaecke, Vera Rogiers, Mathieu Vinken
      Pages 53-76
    5. María Teresa Donato, Laia Tolosa, María José Gómez-Lechón
      Pages 77-93
    6. Mathieu Vinken, Michaël Maes, Sara Crespo Yanguas, Joost Willebrords, Tamara Vanhaecke, Vera Rogiers
      Pages 95-103
    7. Kate Cameron, Baltasar Lucendo-Villarin, Dagmara Szkolnicka, David C. Hay
      Pages 105-111
    8. Joery De Kock, Robim M. Rodrigues, Karolien Buyl, Tamara Vanhaecke, Vera Rogiers
      Pages 113-122
    9. Richard L. Gieseck III, Ludovic Vallier, Nicholas R. F. Hannan
      Pages 123-142
    10. Varvara Gouliarmou, Olavi Pelkonen, Sandra Coecke
      Pages 143-159
    11. Merav Cohen, Gahl Levy, Yaakov Nahmias
      Pages 161-173
    12. Janneke Keemink, Marlies Oorts, Pieter Annaert
      Pages 175-188
    13. Sofia P. Rebelo, Rita Costa, Marcos F. Q. Sousa, Catarina Brito, Paula M. Alves
      Pages 189-202
    14. Jennifer Bolleyn, Joanna Fraczek, Vera Rogiers, Tamara Vanhaecke
      Pages 203-211
  3. In Vitro Methods to Probe Hepatocyte Functionality and Toxicity

    1. Front Matter
      Pages 223-223
    2. Vaibhav Shinde, Regina Stöber, Harshal Nemade, Isaia Sotiriadou, Jürgen Hescheler, Jan Hengstler et al.
      Pages 225-240
    3. Julian Krauskopf, Almudena Espín-Pérez, Jos C. Kleinjans, Theo M. de Kok
      Pages 241-250
    4. Robim M. Rodrigues, Joery De Kock, Tatyana Y. Doktorova, Vera Rogiers, Tamara Vanhaecke
      Pages 279-285
    5. Marc Le Vée, Elodie Jouan, Claire Denizot, Yannick Parmentier, Olivier Fardel
      Pages 287-302
    6. Karolien Buyl, Joery De Kock, Jennifer Bolleyn, Vera Rogiers, Tamara Vanhaecke
      Pages 303-308
    7. Jennifer Bolleyn, Vera Rogiers, Tamara Vanhaecke
      Pages 317-321
    8. Helene Johansson, Ewa C. S. Ellis
      Pages 323-331
    9. Laia Tolosa, María Teresa Donato, María José Gómez-Lechón
      Pages 333-348
    10. Michaël Maes, Tamara Vanhaecke, Bruno Cogliati, Sara Crespo Yanguas, Joost Willebrords, Vera Rogiers et al.
      Pages 349-361
    11. Benjamin L. Woolbright, Hartmut Jaeschke
      Pages 363-376
    12. Gahl Levy, Merav Cohen, Yaakov Nahmias
      Pages 377-390
    13. Luis Perea, Mar Coll, Pau Sancho-Bru
      Pages 391-401
  4. Back Matter
    Pages 403-406

About this book


This volume presents 30 state-of-the-art protocols and reviews to set up and apply primary hepatocyte cultures for research and screening purposes. The first part of the book focuses on the use of these particular liver-based in vitro models to study the different aspects of the hepatocyte life cycle, including cell growth, differentiation and cell death. The second part of the book is targeted towards the demonstration of the applicability of primary hepatocyte cultures, or liver-based in vitro models derived thereof, for functionality and toxicity testing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Protocols In In-Vitro Hepatocyte Research is intended for basic and applied researchers in the area of pharmacology and toxicology, both in academic and industrial settings.   


apoptotic cell death cellular pathways embryonic stem cells hepatocyte functionality heterologous cell-cell in vitro liver bioreactors pluripotent stem cells primary hepatocytes transcriptomic

Editors and affiliations

  • Mathieu Vinken
    • 1
  • Vera Rogiers
    • 2
  1. 1.Department of In Vitro Toxicology and Dermato-CosmetologyVrije Universiteit BrusselBrusselsBelgium
  2. 2.Department of In Vitro Toxicology and Dermato-CosmetologyVrije Universiteit BrusselBrusselsBelgium

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2073-0
  • Online ISBN 978-1-4939-2074-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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