Stem Cell Protocols

  • Ivan N. Rich

Part of the Methods in Molecular Biology book series (MIMB, volume 1235)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Ivan N. Rich
    Pages 1-6
  3. Yi Liu, Gary Van Zant, Ying Liang
    Pages 19-32
  4. Holli Harper, Ivan N. Rich
    Pages 33-48
  5. Bart Vaes, Sara Walbers, Kristel Gijbels, David Craeye, Robert Deans, Jef Pinxteren et al.
    Pages 49-58
  6. Peter A. G. McCourt, Ana Oteiza, Benjamin Cao, Susan K. Nilsson
    Pages 59-71
  7. Jiffin K. Paulose, Edmund B. Rucker III, Vincent M. Cassone
    Pages 73-79
  8. William Hrushesky, Ivan N. Rich
    Pages 81-95
  9. Takamichi Miyazaki, Hirofumi Suemori
    Pages 97-104
  10. Timo Z. Nazari-Shafti, Ivone G. Bruno, Rudy F. Martinez, Michael E. Coleman, Eckhard U. Alt, Scott R. McClure
    Pages 131-146
  11. Yanne S. Doucet, David M. Owens
    Pages 147-164
  12. Matthew J. Branch, Wing-Yan Yu, Carl Sheridan, Andrew Hopkinson
    Pages 165-177
  13. Isobel Massie, Marc Dziasko, Alvena Kureshi, Hannah J. Levis, Louise Morgan, Michael Neale et al.
    Pages 179-202
  14. Seema Parte, Hiren Patel, Kalpana Sriraman, Deepa Bhartiya
    Pages 203-229
  15. Jonathan L. McQualter, Ivan Bertoncello
    Pages 231-241
  16. Eleonora Piscitelli, Cinzia Cocola, Frank Rüdiger Thaden, Paride Pelucchi, Brian Gray, Giovanni Bertalot et al.
    Pages 243-262
  17. Sascha Seidel, Boyan K. Garvalov, Till Acker
    Pages 263-275
  18. Back Matter
    Pages 277-281

About this book


This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.


endothelial cells hematopoietic system stem cell biology stem cells tissue engineering

Editors and affiliations

  • Ivan N. Rich
    • 1
  1. 1.HemoGenix, Inc.Colorado SpringsUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1784-6
  • Online ISBN 978-1-4939-1785-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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