Agrobacterium Protocols

Volume 2

  • Kan Wang

Part of the Methods in Molecular Biology book series (MIMB, volume 1224)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Industrial Plants

    1. Front Matter
      Pages 1-1
    2. Tom Lawrenson, Cassandra Goldsack, Lars Ostergaard, Penny A. C. Hundleby née Sparrow
      Pages 3-10
    3. Keerti S. Rathore, LeAnne M. Campbell, Shanna Sherwood, Eugenia Nunes
      Pages 11-23
    4. Devendra Kumar Maravi, Purabi Mazumdar, Shamsher Alam, Vaibhav V. Goud, Lingaraj Sahoo
      Pages 25-35
    5. Sonia Kapoor, Sanjay S. Parmar, Manju Yadav, Darshna Chaudhary, Manish Sainger, Ranjana Jaiwal et al.
      Pages 37-45
    6. Laura M. Radonic, Dalia M. Lewi, Nilda E. López, H. Esteban Hopp, Alejandro S. Escandón, Marisa López Bilbao
      Pages 47-55
  3. Root Plants

    1. Front Matter
      Pages 57-57
    2. Owen S. D. Wally, Zamir K. Punja
      Pages 59-66
    3. Simon E. Bull
      Pages 67-83
    4. Venkateswari J. Chetty, Javier Narváez-Vásquez, Martha L. Orozco-Cárdenas
      Pages 85-96
    5. Xiaoling He, Susan C. Miyasaka, Maureen M. M. Fitch, Yun J. Zhu
      Pages 97-108
  4. Nuts and Fruits

    1. Front Matter
      Pages 109-109
    2. César Petri, Nuria Alburquerque, Lorenzo Burgos
      Pages 111-119
    3. Guo-Qing Song
      Pages 121-131
    4. Guo-Qing Song
      Pages 133-142
    5. Charles A. Maynard, Linda D. McGuigan, Allison D. Oakes, Bo Zhang, Andrew E. Newhouse, Lilibeth C. Northern et al.
      Pages 143-161
    6. Elena Corredoira, Silvia Valladares, Ana M. Vieitez, Antonio Ballester
      Pages 163-176
    7. Laurent Torregrosa, Sandrine Vialet, Angélique Adivèze, Pat Iocco-Corena, Mark R. Thomas
      Pages 177-194
    8. Satoko Nonaka, Hiroshi Ezura
      Pages 195-203
    9. Silvia Sabbadini, Tiziana Pandolfini, Luca Girolomini, Barbara Molesini, Oriano Navacchi
      Pages 205-215
    10. Roberto Cappelletti, Silvia Sabbadini, Bruno Mezzetti
      Pages 217-227
    11. Charles A. Leslie, Sriema L. Walawage, Sandra L. Uratsu, Gale McGranahan, Abhaya M. Dandekar
      Pages 229-241
  5. Tropic Plants

    1. Front Matter
      Pages 243-243
    2. Vladimir Orbović, Jude W. Grosser
      Pages 245-257
    3. Vladimir Orbović, Alka Shankar, Michael E. Peeples, Calvin Hubbard, Janice Zale
      Pages 259-273
    4. Eveline Déchamp, Jean-Christophe Breitler, Thierry Leroy, Hervé Etienne
      Pages 275-291
    5. Gaurab Gangopadhyay, Kalyan K. Mukherjee
      Pages 293-305
    6. Hao Wu, Fredy Altpeter
      Pages 307-316
  6. Other Important Plants

    1. Front Matter
      Pages 317-317
    2. Mistianne Feeney, Zamir K. Punja
      Pages 319-329
    3. Chia-Wen Li, Chia-Hui Liao, Xia Huang, Ming-Tsair Chan
      Pages 331-346
    4. M. Ashraful Islam, Tage Thorstensen, Jihong Liu Clarke
      Pages 347-356
    5. Quanzi Li, Ting-Feng Yeh, Chenmin Yang, Jingyuan Song, Zenn-Zong Chen, Ronald R. Sederoff et al.
      Pages 357-363
    6. Yaxin Ge, Zeng-Yu Wang
      Pages 365-372
  7. Back Matter
    Pages 373-378

About this book


Rapid changes and significant progress have been made in the Agrobacterium field, such as genetically transforming plants for both basic research purposes and agricultural development.  In Agrobacterium Protocols, Third Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail techniques for delivering DNA to plant cells and permanently altering their genomes.  This edition emphasizes agricultural crops and plant species with economic values, with updated protocols on 32 plant species and protocols involving 19 new species.  Together with the 1st and 2nd editions, these two volumes offer Agrobacterium-mediated genetic transformation protocols for a total of 76 plant species.  For a number of important plants such as rice, barley, wheat and citrus, multiple protocols using different starting plant materials for transformation are included. 


Volume 2 contains 29 chapters with updated techniques for industrial plants, root plants, nuts and fruits, tropic plants, and other important plant species.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, Agrobacterium Protocols, Third Edition facilitates the transfer of this rapidly developing technology to all researchers in both fundamental and applied biology.



Agrobacterium Agrobacterium-mediated genetic transformation DNA molecules DNA to plant cells barley biolistic gun citrus economic values electroporation or polyethylene glycol plant genome plant materials plant species rice transformation wheat

Editors and affiliations

  • Kan Wang
    • 1
  1. 1.Plant Sciences Institute, and Department of AgronomyCenter for Plant Transformation, Iowa State UniversityAmesUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1657-3
  • Online ISBN 978-1-4939-1658-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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