Bacterial Artificial Chromosomes

  • Kumaran Narayanan

Part of the Methods in Molecular Biology book series (MIMB, volume 1227)

Table of contents

  1. Front Matter
    Pages i-xi
  2. BAC Construction and Characterization

    1. Front Matter
      Pages 1-1
    2. Natalay Kouprina, Nicholas C. O. Lee, Artem V. Kononenko, Alexander Samoshkin, Vladimir Larionov
      Pages 3-26
    3. Qingwen Chen, Kumaran Narayanan
      Pages 27-54
    4. Christopher A. Saski, F. Alex Feltus, Laxmi Parida, Niina Haiminen
      Pages 55-67
  3. Modification of BACs

    1. Front Matter
      Pages 69-69
    2. George Kotzamanis, Athanassios Kotsinas
      Pages 123-139
  4. Gene Expression Using BACs as Vectors

  5. Applications of BACs in Model Organisms, Medical Genetics, and Drug Discovery

    1. Front Matter
      Pages 231-231
    2. Jose M. Alonso, Anna N. Stepanova
      Pages 233-243
    3. Petra Kraus, Cecilia L. Winata, Thomas Lufkin
      Pages 245-258
    4. Francesca Romana Grati, François Vialard, Susan Gross
      Pages 259-278
    5. Ahmed B. Hamid, Xiaobo Fan, Nadezda Kosyakova, Gopakumar Radhakrishnan, Thomas Liehr, Tatyana Karamysheva
      Pages 279-287
    6. Kristin Mrasek, Kathleen Wilhelm, Luciana G. Quintana, Luise Theuss, Thomas Liehr, Andreja Leskovac et al.
      Pages 289-298
    7. Anja Weise, Moneeb A. K. Othman, Samarth Bhatt, Sharon Löhmer, Thomas Liehr
      Pages 299-307
    8. Bradley McColl, Sara Howden, Jim Vadolas
      Pages 309-321
    9. Betty R. Kao, Bradley McColl, Jim Vadolas
      Pages 323-343
  6. Back Matter
    Pages 345-348

About this book


Bacterial Artificial Chromosomes, Second Edition expands upon the previous edition with current, detailed methods developed for working with BACs. Updated chapters included in this edition  present fundamental techniques used for BAC construction and characterization, advanced procedures for introducing modifications, achieving gene expression from BAC vectors, applications of BACs in model organisms, and medical genetics and drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step reproducible laboratory protocols, and tips to troubleshoot and avoid known pitfalls.


Authoritative and cutting-edge, Bacterial Artificial Chromosomes, Second Edition seeks to aid scientists in advancing their research using these exciting BAC techniques and strategies.


BAC construction BACs BACs in model organisms achieving gene expression drug discovery medical genetics

Editors and affiliations

  • Kumaran Narayanan
    • 1
  1. 1.Monash University MalaysiaBandar SunwayMalaysia

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1651-1
  • Online ISBN 978-1-4939-1652-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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