Methods and Protocols

  • Heide Schatten
  • Abraham Eisenstark

Part of the Methods in Molecular Biology book series (MIMB, volume 1225)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Sherry A. Dunbar, Vivette Brown Ritchie, Michaela R. Hoffmeyer, Gunjot S. Rana, Hongwei Zhang
    Pages 1-27
  3. Dora Kaloyanova, Mijke Vogels, Bas W. M. van Balkom, J. Bernd Helms
    Pages 29-45
  4. Belgode N. Harish, Godfred A. Menezes
    Pages 47-61
  5. Frederik Czarniak, Michael Hensel
    Pages 63-79
  6. Francisco Ramos-Morales, Elena Cardenal-Muñoz, Mar Cordero-Alba, Fernando Baisón-Olmo
    Pages 93-104
  7. Masayuki Nakano, Eiki Yamasaki, Joel Moss, Toshiya Hirayama, Hisao Kurazono
    Pages 127-138
  8. Carlos Gamazo, Javier Ochoa-Repáraz, Ibai Tamayo, Ana Camacho, Juan M. Irache
    Pages 139-149
  9. Layla M. Malt, Charlotte A. Perrett, Suzanne Humphrey, Mark A. Jepson
    Pages 165-198
  10. Alexander Kehl, Michael Hensel
    Pages 199-225
  11. Geoffrey Gonzalez-Escobedo, John S. Gunn
    Pages 227-235
  12. Andrea I. Moreno Switt, Alexander Sulakvelidze, Martin Wiedmann, Andrew M. Kropinski, David S. Wishart, Cornelis Poppe et al.
    Pages 237-287
  13. Back Matter
    Pages 289-301

About this book


Salmonella: Methods and Protocols, Second Edition expands upon the previous edition with current, detailed methods on different aspects and landmarks advancing knowledge on salmonella research. With new chapters on molecular assays for detection, identification and serotyping of salmonella, quantitative proteomic identification of host factors involved in salmonella infection, determination of antimicrobial resistance in salmonella, site-directed mutagenesis, chromosomal gene analysis, development of bacterial nanoparticle vaccine, attachment of nanoparticle cargo to biotinylated salmonella for combination bacteriotherapy against tumors, various microscopy methods to analyze salmonella interaction with host cells, in vitro modeling of gallbladder-associated salmonella colonization, and analysis of salmonella phages and prophages. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Salmonella: Methods and Protocols, Second Edition present methods that are of value to investigators in the salmonella field.


Salmonella antimicrobial resistance bacterial nanoparticle vaccine bacteriotherapy against tumors biotinylated Salmonella chromosomal gene analysis host cells in vitro modeling of gallbladder mutagenesis nanoparticle cargo phages and prophages serotyping

Editors and affiliations

  • Heide Schatten
    • 1
  • Abraham Eisenstark
    • 2
  1. 1.Department of Veterinary PathobiologyUniversity of MissouriColumbiaUSA
  2. 2.Cancer Research CenterUniversity of MissouriColumbiaUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1624-5
  • Online ISBN 978-1-4939-1625-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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