Methods and Protocols

  • Manfred Heinlein

Part of the Methods in Molecular Biology book series (MIMB, volume 1217)

Table of contents

  1. Front Matter
    Pages i-x
  2. Overview

    1. Front Matter
      Pages 1-1
    2. Iris Sevilem, Shri Ram Yadav, Ykä Helariutta
      Pages 3-24
  3. Imaging of Plasmodesmata

    1. Front Matter
      Pages 53-53
    2. Deborah A. Barton, Robyn L. Overall
      Pages 55-65
  4. Structural Analysis of Plasmodesmata

    1. Front Matter
      Pages 81-81
    2. Magali S. Grison, Lourdes Fernandez-Calvino, Sébastien Mongrand, Emmanuelle M. F. Bayer
      Pages 83-93
    3. Shannon Modla, Jeffrey L. Caplan, Kirk J. Czymmek, Jung-Youn Lee
      Pages 121-133
  5. Analysis of Plasmodesmata Conductivity and Regulation

  6. Studying Macromolecular Trafficking

    1. Front Matter
      Pages 229-229
    2. Dhinesh Kumar, Huan Chen, Yeonggil Rim, Jae-Yean Kim
      Pages 231-243
    3. Christophe Himber, Patrice Dunoyer
      Pages 275-281
    4. Martin Di Donato, Khalid Amari
      Pages 283-293
    5. Jens Tilsner
      Pages 295-328
    6. Eduardo Peña, Manfred Heinlein, Adrian Sambade
      Pages 329-341
  7. Back Matter
    Pages 343-346

About this book


Plasmodesmata: Methods and Protocols provides a unique compendium of currently applied methods to explore plasmodesmata (PD). Opening with overviews of PD structure and their function during development as well as the role of viruses as models for addressing cellular mechanisms in macromolecular trafficking and movement through PD, the book continues with detailed experimental protocols for imaging PD, the isolation and structural analysis of PD, the analysis of PD conductivity and regulation, and for studying cellular mechanisms by which macromolecules are targeted to PD and transported through the channel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and easy to use, Plasmodesmata: Methods and Protocols delivers detailed information about specific practical procedures in order to enable their reproduction and application in PD-related research in laboratories around the world.


Cell signaling Cell-to-cell communication Macromolecular trafficking PD conductivity and regulation PD imaging Plasmodesmata (PD) Virus models

Editors and affiliations

  • Manfred Heinlein
    • 1
  1. 1.Institut de Biologie Moléculaire des Plantes (IBMP-CNRS)StrasbourgFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1522-4
  • Online ISBN 978-1-4939-1523-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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