Plant Endosomes

Methods and Protocols

  • Marisa S. Otegui

Part of the Methods in Molecular Biology book series (MIMB, volume 1209)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Kateřina Malínská, Adriana Jelínková, Jan Petrášek
    Pages 1-11
  3. Thomas Stanislas, Markus Grebe, Yohann Boutté
    Pages 13-29
  4. Miroslav Ovečka, Irene Lichtscheidl, Jozef Šamaj
    Pages 31-44
  5. Vera Bandmann, Peter Haub, Tobias Meckel
    Pages 45-61
  6. Alexandra Chanoca, Marisa S. Otegui
    Pages 63-80
  7. Niloufer G. Irani, Simone Di Rubbo, Eugenia Russinova
    Pages 107-117
  8. Caiji Gao, Yi Cai, Xiaohong Zhuang, Liwen Jiang
    Pages 119-129
  9. Gregory D. Reynolds, Ben August, Sebastian Y. Bednarek
    Pages 163-177
  10. Koji Kasai, Junpei Takano, Toru Fujiwara
    Pages 203-217
  11. Jinggeng Zhou, Ping He, Libo Shan
    Pages 219-231
  12. Carlos Rubilar-Hernández, Glenn R. Hicks, Lorena Norambuena
    Pages 251-264
  13. Back Matter
    Pages 285-286

About this book


Plant Endosomes: Methods and Protocols explores a collection of protocols and techniques to analyze in vivo trafficking of endocytic/endosomal cargo, including lipids, fluids, proteins, and ligands, ultrastructural features of endosomes by high-pressure freezing/freeze-substitution and electron tomography, as well as protein-protein interactions in the endosomal and endomembrane system. The volume continues with coverage of the sorting defects in the transport of vacuolar storage proteins, function conservation of plant endosomal proteins, endosomal trafficking during plant responses to pathogens, protein composition of endosomes and endocytic vesicles, ubiquitination of endosomal cargo proteins, and the identification of novel endosomal components by chemical genomics and proteomics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Detailed and practical, Plant Endosomes: Methods and Protocols gathers contributions from many leading and emerging plant membrane trafficking researchers in order to promote and facilitate novel studies and ideas in this vital field.


Endocytic vesicles Endomembrane system Endosomal system In vivo trafficking Plant endosomal proteins Vacuolar storage proteins

Editors and affiliations

  • Marisa S. Otegui
    • 1
  1. 1.University of Wisconsin-MadisonMadisonUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1419-7
  • Online ISBN 978-1-4939-1420-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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