Regulatory B Cells

Methods and Protocols

  • Gaetano Vitale
  • Francesca Mion

Part of the Methods in Molecular Biology book series (MIMB, volume 1190)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Purification and Phenotypic Characterization of B Cell Subsets

    1. Front Matter
      Pages 1-1
    2. M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, Rita Carsetti
      Pages 3-16
    3. Adèle de Masson, Hélène Le Buanec, Jean-David Bouaziz
      Pages 45-52
  3. Mechanisms of Immune Suppression by B Cells

    1. Front Matter
      Pages 53-53
    2. Yoshiyuki Mishima, Shunji Ishihara, Jonathan J. Hansen, Yoshikazu Kinoshita
      Pages 71-80
    3. Steven K. Lundy, Matthew W. Klinker
      Pages 81-102
  4. Methods for the Ex Vivo Generation/Expansion of IL-10-Producing B Cells

    1. Front Matter
      Pages 103-103
    2. Elisabeth Calderón-Gómez, Simon Fillatreau
      Pages 105-113
    3. Aurélie Tormo, Jiusheng Deng, Edouard Al-Chami, Sonia Ziouani, Moutih Rafei
      Pages 115-125
    4. Luciën E. P. M. van der Vlugt, Simone Haeberlein, Wouter de Graaf, Tyrill E. D. Martha, Hermelijn H. Smits
      Pages 127-141
    5. Adnan R. Khan, Sylvie Amu, Sean P. Saunders, Padraic G. Fallon
      Pages 143-162
    6. Francesca Mion, Federica Benvenuti, Carlo Pucillo, Gaetano Vitale
      Pages 163-179
  5. Study of Regulatory B Cells in Pathological Settings

    1. Front Matter
      Pages 181-181
    2. Elizabeth C. Rosser, Claudia Mauri
      Pages 183-194
    3. Prabitha Natarajan, Linda A. Guernsey, Craig M. Schramm
      Pages 207-225
    4. Atsushi Nishida, Cindy W. Lau, Emiko Mizoguchi, Atsushi Mizoguchi
      Pages 227-241
    5. Jorge Correale, Tomas Rivero Equiza
      Pages 257-269
    6. Arya Biragyn, Catalina Lee-Chang, Monica Bodogai
      Pages 271-289
  6. Back Matter
    Pages 291-292

About this book


Regulatory B Cells: Methods and Protocols presents the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and authoritative, Regulatory B Cells: Methods and Protocols serves as an ideal guide for immunologists as well as for cell and molecular biologists interested in the intricacies of B cell biology.


Anti-inflammatory cytokines Breg IL-10 Immune regulation Immune suppression Pathophysiology Suppressive function

Editors and affiliations

  • Gaetano Vitale
    • 1
  • Francesca Mion
    • 2
  1. 1.Department of Medical and Biological SciencesUniversity of UdineUdineItaly
  2. 2.Department of Medical and Biological SciencesUniversity of UdineUdineItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1160-8
  • Online ISBN 978-1-4939-1161-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals