Genotoxicity and DNA Repair

A Practical Approach

  • L. María Sierra
  • Isabel Gaivão

Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Genotoxicity Assays

    1. Front Matter
      Pages 1-1
    2. Araceli Pillco, Eduardo de la Peña
      Pages 3-22
    3. Christophe Pagnout, Stéphane Jomini, Pascale Bauda
      Pages 43-58
    4. Lucia Migliore, Sebastiano Di Bucchianico, Chiara Uboldi
      Pages 73-102
    5. Edson Luis Maistro
      Pages 103-113
    6. Ana Paula A. Guimarães, Adriana C. Guimarães, Diego Á. Alcântara, Luiz Raimundo Cunha, Patrícia L. Lima, Marne C. Vasconcellos et al.
      Pages 115-139
    7. Dayton M. Petibone, James D. Tucker, Suzanne M. Morris
      Pages 141-158
    8. Vasily N. Dobrovolsky, Robert H. Heflich
      Pages 169-183
    9. Amaya Azqueta, Andrew R. Collins
      Pages 199-217
    10. Carla Costa, João Paulo Teixeira
      Pages 219-239
    11. Julia Catalán, Satu Suhonen, Anna Huk, Maria Dusinska
      Pages 241-268
    12. L. María Sierra, Erico R. Carmona, Leticia Aguado, Ricard Marcos
      Pages 269-282
    13. Ricard Marcos, L. María Sierra, Isabel Gaivão
      Pages 283-295
    14. Mohamed A. Abdalaziz, Balasubramanyam Annangi, Ricard Marcos
      Pages 297-304
    15. John D. Gingerich, Lynda Soper, Christine L. Lemieux, Francesco Marchetti, George R. Douglas
      Pages 305-321
    16. Tao Chen, Xiaoqing Guo, Martha M. Moore
      Pages 323-342
    17. Kiyoshi Sasaki, Anna Huk, Naouale El Yamani, Noriho Tanaka, Maria Dusinska
      Pages 343-362
  3. DNA Repair Assays

    1. Front Matter
      Pages 363-363
    2. Andrew R. Collins, Amaya Azqueta
      Pages 365-376
    3. Jana Slyskova, Sabine A. S. Langie, Isabel Gaivão, Andrew R. Collins, Amaya Azqueta
      Pages 377-395
    4. Isabel Gaivão, Rubén Rodríguez, L. María Sierra
      Pages 397-412
    5. Elise Fouquerel, Jianfeng Li, Andrea Braganza, Zhongxun Yu, Ashley R. Brown, Xiao-Hong Wang et al.
      Pages 413-447
  4. Others

    1. Front Matter
      Pages 449-449
    2. Henriqueta Louro, Miguel Pinto, Nádia Vital, Ana M. Tavares, Pedro M. Costa, Maria João Silva
      Pages 451-477
  5. Back Matter
    Pages 479-483

About this book


Genotoxicity and DNA Repair: A Practical Approach provides a key reference for determining how to analyze the genotoxic activity of molecules or materials and, at the same time, serves as a useful tool for researchers in the Environmental Mutagenesis and DNA Repair fields. Focused on genotoxicity assays recommended by the “OECD guidelines for the testing of chemicals”, this volume also covers other useful assays, such as some gene mutation assays, the comet assay in different species and applications, and the SMART assays of Drosophila. For all the assays, the book presents brief theoretical introductions to the topics and updated standard and modified step-by-step protocols to perform them. Special emphasis is placed on the analysis of nanoparticles, including an integrative approach analysis. The DNA Repair section includes several assays that provide information on repair activity in vitro and in vivo, as well as recent applications to study DNA repair in humans, cell cultures, and animal models. As a volume in the Methods in Pharmacology and Toxicology series, the chapters contain the kind of detail and key implementation advice that ensures reproducible results in the lab.


Authoritative and invaluable, Genotoxicity and DNA Repair: A Practical Approach aims to aid scientists in their pursuit of forwarding this vital field of study.


DNA repair assays Environmental mutagenesis Gene mutation assays Genotoxic activity Nanoparticles and nanomaterials SMART assays The comet assay Toxicology

Editors and affiliations

  • L. María Sierra
    • 1
  • Isabel Gaivão
    • 2
  1. 1.Universidad de OviedoOviedoSpain
  2. 2.Universidade de Trás-os-Montes e Alto DouroVila RealPortugal

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1067-0
  • Online ISBN 978-1-4939-1068-7
  • Series Print ISSN 1557-2153
  • Series Online ISSN 1940-6053
  • Buy this book on publisher's site
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