Directed Evolution Library Creation

Methods and Protocols

  • Elizabeth M.J. Gillam
  • Janine N. Copp
  • David Ackerley

Part of the Methods in Molecular Biology book series (MIMB, volume 1179)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Point Mutagenesis

    1. Front Matter
      Pages 1-1
    2. Janine N. Copp, Paulina Hanson-Manful, David F. Ackerley, Wayne M. Patrick
      Pages 3-22
    3. Ryota Fujii, Motomitsu Kitaoka, Kiyoshi Hayashi
      Pages 23-29
    4. David L. Alexander, Joshua Lilly, Jaime Hernandez, Jillian Romsdahl, Christopher J. Troll, Manel Camps
      Pages 31-44
    5. Anna Joëlle Ruff, Tsvetan Kardashliev, Alexander Dennig, Ulrich Schwaneberg
      Pages 45-68
    6. Miriam Kaltenbach, Nobuhiko Tokuriki
      Pages 69-81
    7. Elsie M. Williams, Janine N. Copp, David F. Ackerley
      Pages 83-101
    8. Alexander Dennig, Jan Marienhagen, Anna Joëlle Ruff, Ulrich Schwaneberg
      Pages 139-149
    9. D. Dafydd Jones, James A. J. Arpino, Amy J. Baldwin, Matthew C. Edmundson
      Pages 159-172
  3. Recombinatorial Methods

    1. Front Matter
      Pages 173-173
    2. James B. Y. H. Behrendorff, Wayne A. Johnston, Elizabeth M. J. Gillam
      Pages 175-187
    3. Wayne M. Patrick, Monica L. Gerth
      Pages 225-244
    4. Stefan Lutz, Ashley B. Daugherty, Ying Yu, Zhen Qian
      Pages 245-258
  4. Structure-Guided Methods and Computational Tools for the Design and Analysis of Mutant Libraries

    1. Front Matter
      Pages 259-259
    2. Rajni Verma, Tuck Seng Wong, Ulrich Schwaneberg, Danilo Roccatano
      Pages 279-290
    3. Eva Sebestova, Jaroslav Bendl, Jan Brezovsky, Jiri Damborsky
      Pages 291-314
    4. Julian Zaugg, Yosephine Gumulya, Elizabeth M. J. Gillam, Mikael Bodén
      Pages 315-333
    5. Matthew A. Smith, Frances H. Arnold
      Pages 335-343
    6. Matthew A. Smith, Frances H. Arnold
      Pages 345-352
    7. Vanessa E. Cox, Eric A. Gaucher
      Pages 353-363
  5. Back Matter
    Pages 365-369

About this book


Directed Evolution Library Creation: Methods and Protocols, Second Edition presents user-friendly protocols for both proven strategies and cutting-edge approaches for the creation of mutant gene libraries for directed evolution. As well as experimental methods, information on current computational approaches is provided in a user-friendly format that will allow researchers to make informed choices without needing to comprehend the full technical details of each algorithm. Directed evolution has become a fundamental approach for engineering proteins to enhance activity and explore structure-function relationships, and has supported the rapid development of the field of synthetic biology over the last decade. Divided into three convenient sections, topics include point mutagenesis strategies, recombinatorial methods wherein genetic diversity is sourced from multiple parental genes that are combined via either homology-dependent or -independent techniques, and a variety of computational methods to guide the design and analysis of mutant libraries. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.


Authoritative and easily accessible, Directed Evolution Library Creation: Methods and Protocols, Second Edition will serve as a reliable manual for both novice and experienced protein engineers and synthetic biologists and will enable further technical innovation and the exploitation of directed evolution for a deeper understanding of protein design and function.


deletion mutations directed evolution gene variant libraries insertion mutations mutagenesis

Editors and affiliations

  • Elizabeth M.J. Gillam
    • 1
  • Janine N. Copp
    • 2
  • David Ackerley
    • 3
  1. 1.Chemistry and Molecular BiosciencesThe University of QueenslandSt. Lucia, BrisbaneAustralia
  2. 2.Michael Smith Laboratories, University of British ColumbiaVancouverCanada
  3. 3.School of Biological SciencesVictoria UniversityWellingtonNew Zealand

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Springer, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-1052-6
  • Online ISBN 978-1-4939-1053-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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