Plant MAP Kinases

Methods and Protocols

  • George Komis
  • Jozef Šamaj

Part of the Methods in Molecular Biology book series (MIMB, volume 1171)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Expression and Activity of Plant MAPKs

  3. Genetic Manipulation of MAPK Function

    1. Front Matter
      Pages 65-65
    2. Elodie Hudik, Souha Berriri, Heribert Hirt, Jean Colcombet
      Pages 67-77
    3. Christian Hettenhausen, Ian T. Baldwin, Jianqiang Wu
      Pages 79-89
  4. Localization Studies of MAPKs and Their Interactions

    1. Front Matter
      Pages 105-105
    2. Miroslav Ovečka, Olga Šamajová, František Baluška, Jozef Šamaj
      Pages 117-130
    3. Anna Doskočilová, Ivan Luptovčiak, Veronika Smékalová, Jozef Šamaj
      Pages 131-145
    4. Alois Schweighofer, Volodymyr Shubchynskyy, Vaiva Kazanaviciute, Armin Djamei, Irute Meskiene
      Pages 147-158
  5. Analysis of MAPK Substrates

    1. Front Matter
      Pages 159-159
    2. Nobuaki Ishihama, Hiroaki Adachi, Miki Yoshioka, Hirofumi Yoshioka
      Pages 171-181
    3. Lennart Eschen-Lippold, Nicole Bauer, Julia Löhr, Mieder A. T. Palm-Forster, Justin Lee
      Pages 183-192
  6. Large Scale Studies on MAPKs, Substrates, and Interactions

    1. Front Matter
      Pages 193-193
    2. Raksha Singh, Sarmina Dangol, Nam-Soo Jwa
      Pages 195-216
    3. Elizabeth K. Brauer, Sorina C. Popescu, George V. Popescu
      Pages 217-235
    4. Jean Bigeard, Delphine Pflieger, Jean Colcombet, Loïc Gérard, Hakim Mireau, Heribert Hirt
      Pages 237-250
    5. Tomoya Asano, Takumi Nishiuchi
      Pages 251-258
  7. Back Matter
    Pages 259-266

About this book


Mitogen-activated protein kinases (MAPKs) are versatile phosphorylating enzymes which regulate multiple proteins involved in gene expression, cell architecture, plant development and reaction to diverse abiotic and biotic factors. The main aim of Plant MAP Kinases: Methods and Protocols is to provide established and new MAPK protocols adapted to the challenges posed by working with plants. The book contains 19 chapters which encompass a wide array of methods progressively scaling from the single gene, protein or cell level to large-scale arrays of proteomic, phosphoproteomic and interactomic data in order to uncover previously unidentified plant MAPK signaling pathways and to tackle with the challenging task of substrate identification. Techniques for MAPK sequence analysis and subcellular localization helping to identify their substrates and subcellular compartmentalization are also provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Plant MAP Kinases: Methods and Protocols represents a collection of useful plant MAPK protocols written by experts in the field for researchers and students.


Arabidopsis mesophyll protoplasts MAPK phosphorylation/activation MAPK transcriptional regulation function by non-radioactive means genetic tools iTRAQ in vivo imaging interrogate MAPK function phospho-affinity based denaturing SDS-PAGE plant MAPKs real time PCR silencing of MAPKs subcellular visualization virus-induced silencing

Editors and affiliations

  • George Komis
    • 1
  • Jozef Šamaj
    • 2
  1. 1.Palacky University in OlomoucOlomoucCzech Republic
  2. 2.Faculty of SciencePalacky University in OlomoucOlomoucCzech Republic

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0921-6
  • Online ISBN 978-1-4939-0922-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Consumer Packaged Goods