Functions and Protocols

  • Laura Poliseno

Part of the Methods in Molecular Biology book series (MIMB, volume 1167)

Table of contents

  1. Front Matter
    Pages i-x
  2. Introductory Chapters

    1. Front Matter
      Pages 1-1
  3. Methods to Identify Pseudogenes

  4. Methods to Detect Transcribed Pseudogenes of a Parental Gene of Interest

  5. High-Throughput Methods to Detect Transcribed Pseudogenes

    1. Front Matter
      Pages 117-117
    2. Stephen C. M. Tsoi, Michael K. Dyck
      Pages 119-127
    3. Adam Frankish, Jennifer Harrow
      Pages 129-155
  6. High-Throughput Methods to Detect Translated Pseudogenes

    1. Front Matter
      Pages 185-185
    2. Nadia Ucciferri, Silvia Rocchiccioli
      Pages 187-195
  7. Methods to Study Transcribed Pseudogenes

    1. Front Matter
      Pages 197-197
    2. Florian A. Karreth, Ugo Ala, Paolo Provero, Pier Paolo Pandolfi
      Pages 199-212
    3. Per Johnsson, Kevin V. Morris, Dan Grandér
      Pages 213-226
    4. Wen-Ling Chan, Jan-Gowth Chang
      Pages 227-239
  8. Methods to Study Translated Pseudogenes

  9. Methods to Avoid Pseudogene Detection

  10. Back Matter
    Pages 317-319

About this book


Providing a list of methods useful both to those who wish to study pseudogenes and to those who actually want to avoid their inadvertent detection, Pseudogenes: Functions and Protocols explores techniques involving pseudogenic DNA, RNA, and peptides/proteins, once believed to lack any functionality, but now known to be involved in complex regulatory circuits. After a few introductory chapters that overview the functions so far attributed to pseudogenes, this thorough volume delves into methods for pseudogene identification, for the detection of pseudogene transcription and translation, and for the study of the functions of pseudogenic RNA and proteins, as well as methods to avoid pseudogene detection when the focus of the research is their highly homologous parental counterparts.  As part of the highly successful Methods in Molecular Biology series, chapters feature the kind of detailed descriptions and implementation advice that ensures successful results in the lab.


Authoritative and practical, Pseudogenes: Functions and Protocols will contribute to the high interest of the scientific community toward pseudogenes, while stimulating the conception of pseudogene-centered research projects and providing experimental protocols that can facilitate their execution.


Inadvertent detection Non-coding genome Pseudogene identification Pseudogenic DNA and RNA Pseudogenic proteins Regulatory long non-coding RNAs Transcribed pseudogenes

Editors and affiliations

  • Laura Poliseno
    • 1
  1. 1.Istituto Toscano TumoriPisaItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0834-9
  • Online ISBN 978-1-4939-0835-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
Industry Sectors
Health & Hospitals