The TNF Superfamily

Methods and Protocols

  • Jagadeesh Bayry

Part of the Methods in Molecular Biology book series (MIMB, volume 1155)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Zhenzhen Wu, Leslie A. Bruggeman
    Pages 1-14
  3. Yelena L. Pobezinskaya, Zhenggang Liu, Swati Choksi
    Pages 15-22
  4. Riccardo Sibilano, Carlo Pucillo, Barbara Frossi
    Pages 23-30
  5. Johannes Trebing, José Antonio Carmona Arana, Steffen Salzmann, Harald Wajant
    Pages 31-45
  6. Sri Ramulu Elluru, Srini V. Kaveri, Jagadeesh Bayry
    Pages 47-54
  7. Jennifer V. Welser-Alves, Amin Boroujerdi, Ulrich Tigges, Richard Milner
    Pages 81-93
  8. Juyang Kim, Hong R. Cho, Byungsuk Kwon
    Pages 95-108
  9. Hangeun Kim, Ranjit Ray
    Pages 125-132
  10. Sahana Holla, Jamma Trinath, Kithiganahalli Narayanaswamy Balaji
    Pages 133-150
  11. Yury V. Shebzukhov, Anna A. Kuchmiy, Andrei A. Kruglov, Frauke Zipp, Volker Siffrin, Sergei A. Nedospasov
    Pages 151-162
  12. Xavier Romero, Juan D. Cañete, Pablo Engel
    Pages 187-199
  13. Isamu Tsuji, Keiji Iwamoto, Yasushi Shintani
    Pages 201-213
  14. Back Matter
    Pages 223-226

About this book


Exploring these type II trans-membrane proteins, The TNF Superfamily: Methods and Protocols focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Opening with protocols to understand the signaling process of TNF family members, this detailed volume continues with technical examples of investigating the role of TNF family members in physiopathologies, protocols on modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, as well as methodologies for various assays of TNF family members and the production of recombinant molecules.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Practical and ready to use, The TNF Superfamily: Methods and Protocols will aid researchers investigating this key family of proteins, involved in vital processes such as providing signals for activation, differentiation, survival and death of cells, modulation of immune response and inflammation, hematopoiesis, and osteoclastogenesis.


Cell signaling Pathogenesis Physiopathology Recombinant molecules TNF family member proteins Type II trans-membrane proteins

Editors and affiliations

  • Jagadeesh Bayry
    • 1
  1. 1.INSERM Unité 1138ParisFrance

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0668-0
  • Online ISBN 978-1-4939-0669-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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