Yeast Metabolic Engineering

Methods and Protocols

  • Valeria Mapelli

Part of the Methods in Molecular Biology book series (MIMB, volume 1152)

Table of contents

  1. Front Matter
    Pages i-x
  2. Molecular Tools and Technology for Yeast Engineering

    1. Front Matter
      Pages 1-1
    2. Ruchi Saraya, Loknath Gidijala, Marten Veenhuis, Ida J. van der Klei
      Pages 43-62
    3. Michael Felber, Harald Pichler, Claudia Ruth
      Pages 87-111
    4. Ceren Alkım, Burcu Turanlı-Yıldız, Z. Petek Çakar
      Pages 169-183
    5. Oliver Spadiut, Christian Dietzsch, Christoph Herwig
      Pages 185-194
  3. Tools and Technologies for Investigation and Determination of Yeast Metabolic Features

    1. Front Matter
      Pages 195-195
    2. Sónia Carneiro, Rui Pereira, Isabel Rocha
      Pages 197-207
    3. Thiago M. Pais, María R. Foulquié-Moreno, Johan M. Thevelein
      Pages 251-266
  4. Metabolic Models for Yeast Metabolic Engineering

  5. Patenting and Regulations

    1. Front Matter
      Pages 295-295
  6. Back Matter
    Pages 311-316

About this book


Yeast Metabolic Engineering: Methods and Protocols provides the widely established basic tools used in yeast metabolic engineering, while describing in deeper detail novel and innovative methods that have valuable potential to improve metabolic engineering strategies in industrial biotechnology applications. Beginning with an extensive section on molecular tools and technology for yeast engineering, this detailed volume is not limited to methods for Saccharomyces cerevisiae, but describes tools and protocols for engineering other yeasts of biotechnological interest, such as Pichia pastoris, Hansenula polymorpha, and Zygosaccharomyces bailii. Tools and technologies for the investigation and determination of yeast metabolic features are described in detail, as well as metabolic models and their application for yeast metabolic engineering, while a chapter describing patenting and regulations with a special glance at yeast biotechnology closes the volume.  Written in the highly successful Methods in Molecular Biology series format, most chapters include an introduction to their respective topic, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and authoritative, Yeast Metabolic Engineering: Methods and Protocols aims to familiarize researchers with the current state of these vital and increasingly useful technologies.


Bioinformatics tools Genetic engineering Hansenula polymorpha Industrial biotechnology applications Komagataella spp. Metabolic models Pichia pastoris Saccharomyces cerevisiae Yeast metabolism Yeast metabolome analysis Zygosaccharomyces bailii

Editors and affiliations

  • Valeria Mapelli
    • 1
  1. 1.Industrial BiotechnologyChalmers University of TechnologyGothenburgSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media, LLC 2014
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-0562-1
  • Online ISBN 978-1-4939-0563-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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